TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation
TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation
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DOI：
10.3748/wjg.v13.i19.2717
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发表时间：
2007-05-21
影响因子：
4.3
通讯作者：
Kusunoki, Masato
中科院分区：
文献类型：
--
作者：
Toiyama, Yuji;Mizoguchi, Akira;Kusunoki, Masato
目的：研究TTYH 2在人结肠癌及结肠癌细胞系中的表达模式，并探讨小干扰RNA（siRNA）对TTYH 2在结肠癌细胞系中表达的抑制作用。此外，构建了针对TTYH 2的siRNA质粒表达载体，并用Lipofectamine（TM）2000转染到DLD-1和Caco-2中。结果：TTYH 2基因在结肠癌组织中的表达明显高于正常结肠黏膜（1.23 ± 0.404vs0.655 ± 0.373，P = 0.0103），而在正常结肠黏膜中TTYH 2基因的表达明显低于正常结肠黏膜（1.23 ± 0.404vs0.655 ± 0.373，P = 0.0103）。结肠癌衍生的细胞系包括DLD-1、Caco-2和Lovo也表达高水平的TTYH 2。与此相反，转染siRNA-TTYH 2显着抑制这些癌细胞株的增殖和散射。结论：目前的工作表明，第一次，TTYH 2基因表达显着上调，在结肠癌。TTYH 2基因可能在调节大肠癌的增殖和转移潜能中起重要作用。(C)2007年，WJG出版社。All rights reserved.
AIM: To investigate the expression patterns of TTYH2 in the human colon cancer and colon cancer cell lines and to evaluate the inhibitory effect of small interfering RNA (siRNA) on the expression of TTYH2 in colon cancer cell lines.METHODS: We investigated the expression patterns of TTYH2 in colon cancer, adjacent non-tumorous colon mucosa, and cancer cell lines (DLD-1, caco-2, and Lovo) by RT-PCR. Furthermore, a siRNA plasmid expression vector against TTYH2 was constructed and transfected into DLD-1 and Caco-2 with Lipofectamine(TM) 2000. The down regulation of TTYH2 expression was detected by RT-PCR and the role of siRNA in inducing cell proliferation and cell aggregation was evaluated by MTT and aggregation assay.RESULTS: TTYH2 gene expression in colon cancer tissue was significantly up-regulated compared with normal colonic mucosa (1.23 +/- 0.404 vs 0.655 +/- 0.373, P = 0.0103). Colon cancer derived cell lines including DLD-1, Caco-2, and Lovo also expressed high levels of TTYH2. In contrast, transfection with siRNA-TTYH2 significantly inhibited both proliferation and scattering of these cancer cell lines.CONCLUSION: The present work demonstrates, for the first time, that the TTYH2 gene expression is significantly up-regulated in colon cancer. The TTYH2 gene may play an important role in regulating both proliferating and metastatic potentials of colorectal cancer. (C) 2007 The WJG Press. All rights reserved.