ACCELERATION OF THE ONSET OF DIABETES IN NOD MICE BY THYMECTOMY AT WEANING
ACCELERATION OF THE ONSET OF DIABETES IN NOD MICE BY THYMECTOMY AT WEANING
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DOI：
10.1002/eji.1830190516
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发表时间：
1989-05-01
影响因子：
5.4
通讯作者：
BACH, JF
中科院分区：
文献类型：
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作者：
DARDENNE, M;LEPAULT, F;BACH, JF
在非肥胖糖尿病（NOD）小鼠中检查了在断奶（3周）和6-7周龄时进行的胸腺切除术对糖尿病发病率的影响，所述非肥胖糖尿病（NOD）小鼠是类似于人I型糖尿病的免疫介导的胰岛素依赖性糖尿病的自发模型。当在断奶时进行，胸腺切除导致糖尿病的发病率显着增加，在NOD女性相比，假胸腺切除动物。相反，在NOD男性中进行胸腺切除术时，注意到疾病的发病率或胰岛炎的表达没有变化。当延迟超过6-7周龄时，胸腺切除术对NOD雄性和雌性没有影响。对来自完整小鼠和在断奶时或在6-7周龄时切除胸腺的小鼠的脾细胞的流式细胞术分析表明，在两组切除胸腺的动物中T细胞亚群显著耗竭。这些结果表明，在NOD小鼠糖尿病的发病提交胸腺调节和断奶时胸腺切除术诱导的T细胞耗竭加速疾病，效果可能是由于一些T细胞依赖性抑制机制的损失。
The effect of thymectomy performed at weaning (3 weeks) and at 6-7 weeks of age on the incidence of diabetes was examined in the non-Obese diabetic (NOD) mouse, a spontaneous model of immunologically mediated insulin-dependent diabetes similar to human type I diabetes. When performed at weaning, thymectomy led to a dramatic increase in the incidence of diabetes in NOD females in comparison to sham-thymectomized animals. Conversely, no change in the incidence of the disease or the expression of insulitis was noted when thymectomy was performed in NOD males. When delayed beyond 6-7 weeks of age, thymectomy had no effect on NOD males and females. Flow cytometry analysis of spleen cells from intact mice and mice thymectomized at weaning or at 6-7 weeks of age demonstrated a significant depletion of the T cell subsets in both groups of thymectomized animals. These results indicate that the onset of diabetes in NOD mice is submitted to thymic regulation and that the T cell depletion induced by thymectomy at weaning accelerates the disease, an effect possibly due to the loss of some T cell-dependent suppressor mechanisms.