Time-dependent toxicity of cadmium telluride quantum dots on liver and kidneys in mice: histopathological changes with elevated free cadmium ions and hydroxyl radicals.
Time-dependent toxicity of cadmium telluride quantum dots on liver and kidneys in mice: histopathological changes with elevated free cadmium ions and hydroxyl radicals.
复制标题
碲化镉量子点对小鼠肝脏和肾脏的时间依赖性毒性：游离镉离子和羟自由基升高的组织病理学变化
DOI：
10.2147/ijn.s103489
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发表时间：
2016
影响因子：
8
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Wang M;Wang J;Sun H;Han S;Feng S;Shi L;Meng P;Li J;Huang P;Sun Z
全面了解量子点在体内的毒理学行为是其应用于人体的前提和重要条件。与众多研究量子点的细胞毒性研究相比，只有少数量子点的体内研究被报道，并且这个问题仍然存在争议。我们的研究旨在了解QD在不同时间点介导的毒性，并探讨游离镉离子（Cd 2+）和羟基自由基（·OH）在组织损伤中的作用。雄性ICR小鼠单次静脉注射CdTe QD（1.5 µmol/kg），随后在第1、7、14和28天检查肝脏和肾脏功能及形态。此外，通过用水杨酸（SA）作为2，3-二羟基苯甲酸（DHBA）捕获· OH并使用高效液相色谱荧光法检测来定量组织中·OH的产生。我们使用组织金属硫蛋白水平和2，3-DHBA：SA比率的诱导作为标记物，分别从组织中的量子点降解和·OH产生中升高的Cd 2+。实验结果表明，QD诱导的组织病理学变化具有时间依赖性，随着Cd 2+和·OH浓度的升高，组织病理学变化可在一段时间后恢复。Cd ~（2+）和·OH在组织病理学异常方面表现出延迟效应。在多个时间点进行的组织学评估可能有助于评价QD的生物安全性。
A complete understanding of the toxicological behavior of quantum dots (QDs) in vivo is of great importance and a prerequisite for their application in humans. In contrast with the numerous cytotoxicity studies investigating QDs, only a few in vivo studies of QDs have been reported, and the issue remains controversial. Our study aimed to understand QD-mediated toxicity across different time points and to explore the roles of free cadmium ions (Cd2+) and hydroxyl radicals (·OH) in tissue damage. Male ICR mice were administered a single intravenous dose (1.5 µmol/kg) of CdTe QDs, and liver and kidney function and morphology were subsequently examined at 1, 7, 14, and 28 days. Furthermore, ·OH production in the tissue was quantified by trapping · OH with salicylic acid (SA) as 2,3-dihydroxybenzoic acid (DHBA) and detecting it using a high-performance liquid chromatography fluorescence method. We used the induction of tissue metallothionein levels and 2,3-DHBA:SA ratios as markers for elevated Cd2+ from the degradation of QDs and ·OH generation in the tissue, respectively. Our experimental results revealed that the QD-induced histopathological changes were time-dependent with elevated Cd2+ and ·OH, and could recover after a period of time. The Cd2+ and ·OH exhibited delayed effects in terms of histopathological abnormalities. Histological assessments performed at multiple time points might facilitate the evaluation of the biological safety of QDs.