Calcium-binding protein S100P is a novel diagnostic marker of cholangiocarcinoma
Calcium-binding protein S100P is a novel diagnostic marker of cholangiocarcinoma
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DOI：
10.1111/j.1349-7006.2010.01757.x
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发表时间：
2011-01-01
期刊：
影响因子：
5.7
通讯作者：
Shimosegawa, Tooru
中科院分区：
文献类型：
--
作者：
Hamada, Shin;Satoh, Kennichi;Shimosegawa, Tooru
尽管诊断方法有所改进，但胆管癌的发病率和死亡率仍在增加。由于刷细胞学检查对胆管癌的敏感性并不令人满意，因此需要建立一种新的诊断标志物。最近的一份报告表明胆管癌中钙结合蛋白 S100P 的上调。采用免疫组织化学方法评估正常胆管和胆管癌组织中S100P的表达状态。通过实时逆转录聚合酶链式反应 (RT-PCR) 评估良性胆管狭窄和胆管癌内镜逆行胰胆管造影 (ERCP) 过程中刷检细胞学样本中 S100P mRNA 的表达水平。比较每种诊断策略的敏感性和特异性。 S100P在胆管癌组织中频繁表达，但在正常胆管中不表达。胆管癌病例的刷检细胞学样本显示，与良性胆道狭窄相比，S100P 的表达水平更高。 S100P的相对表达水平能够以比经典细胞学更高的敏感性来判断胆管癌，并且S100P表达水平与细胞学相结合的敏感性为90.0%，特异性为92.0%。钙结合蛋白S100P是胆管癌的新标志物。检测刷细胞学样本中S100P的表达水平具有诊断价值，有助于更好地诊断胆管癌。 （《癌症科学》2011 年；102：150-156）。
The incidence and mortality of cholangiocarcinoma are increasing despite improvements in the diagnostic method. Since the sensitivity of brushing cytology for cholangiocarcinoma is not satisfactory, a novel diagnostic marker needs to be established. A recent report has suggested upregulation of the calcium-binding protein S100P in cholangiocarcinoma. The expression status of S100P in normal bile duct and cholangiocarcinoma tissues was assessed by immunohistochemistry. The expression levels of S100P mRNA in the brushing cytology samples during endoscopic retrograde cholangiopancreatography (ERCP) from benign biliary strictures and cholangiocarcinoma were assessed by real-time reverse transcription-polymerase chain reaction (RT-PCR). The sensitivity and specificity of each diagnostic strategy was compared. S100P was frequently expressed in the cholangiocarcinoma tissues, but not in the normal bile duct. The brushing cytology samples from the cholangiocarcinoma cases revealed higher expression levels of S100P compared with the benign biliary strictures. The relative expression level of S100P could determine the cholangiocarcinoma at higher sensitivity than classical cytology, and the combination of the S100P expression level and cytology yielded a sensitivity of 90.0%, with a specificity of 92.0%. Calcium-binding protein S100P is a novel marker of cholangiocarcinoma. Detecting the S100P expression levels in brushing cytology samples has a diagnostic value, which will be helpful for better diagnosis of cholangiocarcinoma. (Cancer Sci 2011; 102: 150-156).