Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane
Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane
复制标题
干燥增强 PSII 磷酸化并影响类囊体膜中蛋白质复合物的分布
DOI：
10.1111/ppl.12258
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发表时间：
2015-03-01
影响因子：
6.4
通讯作者：
Wang, Guangce
中科院分区：
文献类型：
--
作者：
Gao, Shan;Gu, Wenhui;Wang, Guangce
脱水对潮间带大型藻类的光合过程有显著影响。我们研究了一种能耐受脱水的潮间带大型藻类——石莼属（Ulva sp.），以探究其光合性能以及类囊体膜蛋白的成分和结构在脱水响应中的变化。我们的研究结果表明，在石莼属中，光系统II（PSII）比光系统I（PSI）对脱水更敏感。对不同脱水程度下类囊体膜蛋白的比较蛋白质组学研究表明，在脱水过程中，参与光合作用的蛋白质含量变化不大。有趣的是，我们发现PSII亚基PsbS（光系统II S亚基）（LHC超家族中的一种四螺旋蛋白）以及与光捕获复合物应激相关的（LHCSR）蛋白，它们分别是陆生植物和藻类中非光化学猝灭所必需的，在正常和脱水条件下均存在，且在脱水过程中均略有增加。此外，免疫印迹分析结果表明，在脱水过程中PSII和LHCII的磷酸化增加。为了进一步研究，我们通过蓝色天然 - 聚丙烯酰胺凝胶电泳分离出了脱水过程中形成的一种超复合物，并通过质谱分析确定了其成分。我们的研究结果表明，随着含水量的降低，该复合物的磷酸化略有增加。所有结果表明，在脱水过程中，潮间带大型藻类石莼属的类囊体膜蛋白含量变化不大，但蛋白质复合物发生了重排。
Desiccation has significant effects on photosynthetic processes in intertidal macro-algae. We studied an intertidal macro-alga, Ulva sp., which can tolerate desiccation, to investigate changes in photosynthetic performance and the components and structure of thylakoid membrane proteins in response to desiccation. Our results demonstrate that photosystem II (PSII) is more sensitive to desiccation than photosystem I (PSI) in Ulva sp. Comparative proteomics of the thylakoid membrane proteins at different levels of desiccation suggested that there were few changes in the content of proteins involved in photosynthesis during desiccation. Interestingly, we found that both the PSII subunit, PsbS (Photosystem II S subunit) (a four-helix protein in the LHC superfamily), and light-harvesting complex stress-related (LHCSR) proteins, which are required for non-photochemical quenching in land plants and algae, respectively, were present under both normal and desiccation conditions and both increased slightly during desiccation. In addition, the results of immunoblot analysis suggested that the phosphorylation of PSII and LHCII increases during desiccation. To investigate further, we separated out a supercomplex formed during desiccation by blue native-polyacrylamide gel electrophoresis and identified the components by mass spectrometry analysis. Our results show that phosphorylation of the complex increases slightly with decreased water content. All the results suggest that during the course of desiccation, few changes occur in the content of thylakoid membrane proteins, but a rearrangement of the protein complex occurs in the intertidal macro-alga Ulva sp.