Multispecies reconstructions uncover widespread conservation, and lineage-specific elaborations in eukaryotic mRNA metabolism
Multispecies reconstructions uncover widespread conservation, and lineage-specific elaborations in eukaryotic mRNA metabolism
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DOI：
10.1371/journal.pone.0192633
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发表时间：
2018-03-21
期刊：
影响因子：
3.7
通讯作者：
Carrington, Mark
中科院分区：
文献类型：
--
作者：
Bannerman, Bridget P.;Kramer, Susanne;Carrington, Mark
真核生物细胞质mRNA代谢途径的保守和进化程度仍然没有完全解决。在这项研究中，我们描述了一个全面的基因组和转录组范围内的蛋白质参与mRNA成熟，翻译和mRNA衰变的代表性生物从六个真核生物超组。我们证明，真核生物共享的mRNA代谢，几乎可以肯定存在于最后的真核生物共同祖先的共同途径，并显示第一次内含子密度和选择性缺乏一些外显子连接复合物（EJC）组件在真核生物之间的相关性。此外，我们确定了在个别谱系中多样化的途径，特别关注的是，与其他生物体相比，挖掘动物和SAR组的成员中独特的基因获得和损失，这有助于其独特的基因表达途径。
The degree of conservation and evolution of cytoplasmic mRNA metabolism pathways across the eukaryotes remains incompletely resolved. In this study, we describe a comprehensive genome and transcriptome-wide analysis of proteins involved in mRNA maturation, translation, and mRNA decay across representative organisms from the six eukaryotic super-groups. We demonstrate that eukaryotes share common pathways for mRNA metabolism that were almost certainly present in the last eukaryotic common ancestor, and show for the first time a correlation between intron density and a selective absence of some Exon Junction Complex (EJC) components in eukaryotes. In addition, we identify pathways that have diversified in individual lineages, with a specific focus on the unique gene gains and losses in members of the Excavata and SAR groups that contribute to their unique gene expression pathways compared to other organisms.