Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding
Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding
复制标题
开发单倍体诱导剂介导的基因组编辑系统以加速玉米育种
DOI：
10.1016/j.molp.2019.03.006
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发表时间：
2019-04-01
期刊：
影响因子：
27.5
通讯作者：
Wang, Haiyang
中科院分区：
文献类型：
--
作者：
Wang, Baobao;Zhu, Lei;Wang, Haiyang
作物育种的目的是产生具有多种期望性状的纯近交系。双单倍体（DH）和使用CRISPR/Cas9的基因组编辑是作物育种中两种强大的改变游戏规则的技术。但这两个品系都不适合于快速选育优良性状综合的纯系。在这里，我们报告了一种单倍体诱导物介导的基因组编辑（IMGE）方法的开发，该方法利用携带靶向所需农艺性状的CRISPR/Cas9盒的玉米单倍体诱导物系来为优良玉米自交系授粉并在优良玉米背景中产生基因组编辑的单倍体。具有所需性状改良的纯合纯DH系可以在两代内产生，从而绕过了常规育种中用于将所需性状整合到精英商业背景中的重复杂交和回交的冗长程序。
Crop breeding aims to generate pure inbred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize inbred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds.