Dietary selenium affects host selenoproteome expression by influencing the gut microbiota
Dietary selenium affects host selenoproteome expression by influencing the gut microbiota
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DOI：
10.1096/fj.11-181990
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发表时间：
2011-07-01
期刊：
影响因子：
4.8
通讯作者：
Gladyshev, Vadim N.
中科院分区：
文献类型：
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作者：
Kasaikina, Marina V.;Kravtsova, Marina A.;Gladyshev, Vadim N.
胃肠道的定植和微生物群的组成可能受到饮食成分的影响，包括微量元素。为了了解硒如何调节肠道菌群，我们使用高通量测序来检查维持在缺硒，硒充足和富硒饮食的小鼠肠道菌群的组成。由于饮食中的硒，微生物群多样性增加。特定的微生物类型显示出硒的不同作用，即使在一个属内，这意味着硒在微生物类群中具有独特的作用。传统的无菌小鼠硒饮食得到了类似的结果，并显示出增加的细菌种群的多样性，在动物喂养更高水平的硒。无菌小鼠喂养硒饮食修改其selenoproteome表达类似于对照小鼠，但表现出较高的水平和活性的谷胱甘肽过氧化物酶1和蛋氨酸-R-亚砜还原酶1在肝脏中，这表明部分螯合硒的肠道微生物，限制其可用性的主机。硒状态的这些变化与其他微量元素的水平无关。数据显示，膳食硒影响肠道微生物菌群的组成和胃肠道的定植，这反过来又影响宿主硒状态和硒蛋白质组的表达。Kasaikina，M.五、Kravtsova，M.一、李，B。C.的方法，Seravalli，J.，彼得森，D.一、沃尔特，J.，莱格河，Benson，A. K.，Hatfield，D. L.，格拉迪舍夫膳食硒通过影响肠道菌群影响宿主硒蛋白质组的表达。FASEB J.25，2492-2499（2011）. www.fasebj.org
Colonization of the gastrointestinal tract and composition of the microbiota may be influenced by components of the diet, including trace elements. To understand how selenium regulates the intestinal microflora, we used high-throughput sequencing to examine the composition of gut microbiota of mice maintained on selenium-deficient, selenium-sufficient, and selenium-enriched diets. The microbiota diversity increased as a result of selenium in the diet. Specific phylotypes showed differential effects of selenium, even within a genus, implying that selenium had unique effects across microbial taxa. Conventionalized germfree mice subjected to selenium diets gave similar results and showed an increased diversity of the bacterial population in animals fed with higher levels of selenium. Germ-free mice fed selenium diets modified their selenoproteome expression similar to control mice but showed higher levels and activity of glutathione peroxidase 1 and methionine-R-sulfoxide reductase 1 in the liver, suggesting partial sequestration of selenium by the gut microorganisms, limiting its availability for the host. These changes in the selenium status were independent of the levels of other trace elements. The data show that dietary selenium affects both composition of the intestinal microflora and colonization of the gastrointestinal tract, which, in turn, influence the host selenium status and selenoproteome expression.-Kasaikina, M. V., Kravtsova, M. A., Lee, B. C., Seravalli, J., Peterson, D. A., Walter, J., Legge, R., Benson, A. K., Hatfield, D. L., Gladyshev, V. N. Dietary selenium affects host selenoproteome expression by influencing the gut microbiota. FASEB J. 25, 2492-2499 (2011). www.fasebj.org