Regenerative activity and liver function following partial hepatectomy in the rat using 31P-MR spectroscopy
Regenerative activity and liver function following partial hepatectomy in the rat using 31P-MR spectroscopy
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DOI：
10.1053/jhep.2002.34742
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发表时间：
2002-08-01
期刊：
影响因子：
13.5
通讯作者：
Minuk, GY
中科院分区：
文献类型：
--
作者：
Corbin, IR;Buist, R;Minuk, GY
本研究的目的是确定肝部分切除术（PHx）后肝脏能量消耗的改变，如体内肝脏P-31-MRS所记录的，是否与肝再生和/或肝功能的标准参数相关。此外，我们试图确定肝脏能量水平的变化是否与肝切除的程度成比例。成年雄性Sprague-Dawley大鼠（每组4-7只）接受40%、70%或90% PHx或假手术。24或48小时后，对每只动物进行磁共振波谱（MRS）检查。MRS检查后，对每只大鼠进行[H-3]胸苷掺入肝DNA、增殖细胞核抗原（PCNA）蛋白表达和血清胆红素测定。手术后24小时，接受70%PHx的大鼠三磷酸腺苷（ATP）水平不变，但ATP/无机磷酸盐（Pi）比值显著降低（P <0.05），而在PHx后48小时，ATP和ATP/Pi水平均低于假手术和非手术对照组（P <0.05）。肝再生和肝功能损害反映了这些变化，ATP/Pi比值与[H-3]胸苷掺入（r =-0.61，P < .005）、PCNA蛋白表达（r =-0.62，P < .005）和血清胆红素（r =-0.49，P < .05）相关。对于已经经历分级切除的大鼠，PHx后48小时的耗尽能量水平与切除的程度、增强的再生活性的程度和肝功能障碍成比例。总之，P-31-MRS生成的ATP/Pi指数是一种非侵入性的，稳健的测定，与肝再生和功能的标准参数相关。
The aim of the present study was to determine whether alterations in hepatic energy expenditure following partial hepatectomy (PHx), as documented by in vivo hepatic P-31-MRS, correlate with standard parameters of hepatic regeneration and/or liver function. In addition, we sought to determine whether changes in hepatic energy levels are proportional to the extent of hepatic resection. Adult male Sprague-Dawley rats (4-7 per group) underwent a 40%, 70%, or 90% PHx or sham surgeries. Magnetic resonance spectroscopy (MRS) examinations were performed on each animal 24 or 48 hours thereafter. After MRS examinations, [H-3]thymidine incorporation into hepatic DNA, proliferating cell nuclear antigen (PCNA) protein expression, and serum bilirubin determinations were performed on each rat. Twenty-four hours following surgery, rats that had undergone 70% PHx had unchanged adenosine triphosphate (ATP) levels but significantly lower ATP/inorganic phosphate (Pi) ratios (P < .05), whereas, at 48 hours post-PHx, both ATP and ATP/Pi levels were lower than in sham- and nonoperated controls (P < .05). Hepatic regeneration and liver dysfunction mirrored these changes; correlations existed between ATP/Pi ratios and [H-3] thymidine incorporation (r = -0.61, P < .005), PCNA protein expression (r = -0.62, P < .005), and serum bilirubin (r = -0.49, P < .05). For rats that had undergone graded resections, depleted energy levels 48 hours post-PHx were proportional to the extent of resection, degree of enhanced regenerative activity, and liver dysfunction. In conclusion, P-31-MRS-generated ATP/Pi index is a noninvasive, robust determination that correlates with standard parameters of hepatic regeneration and function.