Isolation and physiological properties of methanogenic archaea that degrade tetramethylammonium hydroxide
Isolation and physiological properties of methanogenic archaea that degrade tetramethylammonium hydroxide
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DOI：
10.1007/s00253-023-12488-2
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发表时间：
2023-03
影响因子：
5
通讯作者：
Akinori Iguchi;Yasuyuki Takemura;Tsuyoshi Danshita;Takuya Kurihara;Masataka Aoki;S. Hori;T. Shigematsu;Kazuaki Syutsubo
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文献类型：
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作者：
Akinori Iguchi;Yasuyuki Takemura;Tsuyoshi Danshita;Takuya Kurihara;Masataka Aoki;S. Hori;T. Shigematsu;Kazuaki Syutsubo
四甲基氢氧化铵（TMAH）是一种已知的有毒化学品，用于半导体光电子工艺的光刻工艺。电子工业排放大量含有TMAH的废水。因此，将厌氧处理应用于含TMAH的工业废水具有吸引力。在这项研究中，一个新的TMAH降解产甲烷古菌分离的颗粒污泥的嗜冷上流式厌氧污泥床（UASB）反应器处理合成废水含有TMAH。虽然该菌株（NY-STAYD）与Methanomethylovorans uponensis有亲缘关系，但它是唯一分离到的能降解TMAH的Methanomethylovorans菌株。菌株NY-STAYD具有降解甲胺类化合物的能力，与以前分离的Methanomethylovoransspp.虽然该菌株能够在15至37°C的温度范围内生长，但细胞产量在较低温度下更高。利用16 SrRNA特异性寡核苷酸探针荧光原位杂交（FISH）技术，研究了甲烷甲基噬菌体（Methanomethylovorans）属古菌细胞在原颗粒污泥中的分布。研究结果表明，TMAH降解菌Methanomethylovorans并没有与其它微生物混合，而是作为一个单独的优势古菌分离在颗粒表面。关键点·分离到一株TMAH降解菌Methanomethylovorans·该菌株是目前已知的唯一一株能降解TMAH的Methanomethylovorans菌株·该菌株在低温条件下的细胞产量最高
Tetramethylammonium hydroxide (TMAH) is a known toxic chemical used in the photolithography process of semiconductor photoelectronic processes. Significant amounts of wastewater containing TMAH are discharged from electronic industries. It is therefore attractive to apply anaerobic treatment to industrial wastewater containing TMAH. In this study, a novel TMAH-degrading methanogenic archaeon was isolated from the granular sludge of a psychrophilic upflow anaerobic sludge blanket (UASB) reactor treating synthetic wastewater containing TMAH. Although the isolate (strain NY-STAYD) was phylogenetically related toMethanomethylovorans uponensis, it was the only isolatedMethanomethylovoransstrain capable of TMAH degradation. Strain NY-STAYD was capable of degrading methylamine compounds, similar to the previously isolatedMethanomethylovoransspp. While the strain was able to grow at temperatures ranging from 15 to 37°C, the cell yield was higher at lower temperatures. The distribution of archaeal cells affiliated with the genusMethanomethylovoransin the original granular sludge was investigated by fluorescence in situ hybridization (FISH) using specific oligonucleotide probe targeting 16S rRNA. The results demonstrated that the TMAH-degrading cells associated with the genusMethanomethylovoranswere not intermingled with other microorganisms but rather isolated on the granule’s surface as a lone dominant archaeon.Key points• A TMAH-degrading methanogenic Methanomethylovorans strain was isolated• This strain was the only known Methanomethylovorans isolate that can degrade TMAH• The highest cell yield of the isolate was obtained at psychrophilic conditionsGraphical Abstract