A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.
A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing.
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DOI：
10.1016/j.stemcr.2015.02.007
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发表时间：
2015-04-14
影响因子：
5.9
通讯作者：
Loewke, Kevin E.
中科院分区：
文献类型：
--
作者：
Maddah, Mahnaz;Heidmann, Julia D.;Mandegar, Mohammad A.;Walker, Chase D.;Bolouki, Sara;Conklin, Bruce R.;Loewke, Kevin E.
我们提出了一种基于视频显微镜和图像分析的非侵入性方法来表征多能干细胞来源的心肌细胞的功能。该平台名为Pulse，基于以快速帧速率捕获的相衬图像的运动分析，自动测量心跳频率、心跳持续时间、幅度和心跳之间的变化。使用Pulse，我们展示了在干细胞来源的心肌细胞中不使用外源标记的药物效应的重现，并表明我们的平台可以用于高通量心脏毒性药物的筛选和生理相关表型的研究。应用视频运动分析对心肌细胞的非侵入性表征标准平板中单细胞、单层或组织心肌细胞的评估药物筛选在本文中，Maddah及其同事提出了一种基于视频显微镜和图像分析的非侵入性方法来表征干细胞来源的心肌细胞的功能。他们展示了心脏搏动频率、持续时间、幅度和变异的自动测量，并表明该平台可用于心脏毒性药物筛选和研究生理相关表型。
We present a non-invasive method to characterize the function of pluripotent stem-cell-derived cardiomyocytes based on video microscopy and image analysis. The platform, called Pulse, generates automated measurements of beating frequency, beat duration, amplitude, and beat-to-beat variation based on motion analysis of phase-contrast images captured at a fast frame rate. Using Pulse, we demonstrate recapitulation of drug effects in stem-cell-derived cardiomyocytes without the use of exogenous labels and show that our platform can be used for high-throughput cardiotoxicity drug screening and studying physiologically relevant phenotypes. Non-invasive characterization of cardiomyocytes using video motion analysis Assessment of single-cell, monolayer, or tissue cardiomyocytes in standard plates Drug screening by a label-free, contact-free imaging assay In this article, Maddah and colleagues present a non-invasive method to characterize the function of stem-cell-derived cardiomyocytes based on video microscopy and image analysis. They demonstrate automated measurements of beating frequency, duration, amplitude, and variation and show that the platform can be used for cardiotoxicity drug screening and studying physiologically relevant phenotypes.
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作者：
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通讯作者：
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DOI：
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