Early embryonic interaction of retinal pigment epithelium and mesenchymal tissue induces conversion of pigment epithelium to neural retinal fate in the silver mutation of the Japanese quail
Early embryonic interaction of retinal pigment epithelium and mesenchymal tissue induces conversion of pigment epithelium to neural retinal fate in the silver mutation of the Japanese quail
复制标题
视网膜色素上皮和间质组织的早期胚胎相互作用诱导日本鹌鹑银突变色素上皮向神经视网膜命运的转化
DOI：
--
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
M. Tsudzuki
中科院分区：
文献类型：
--
作者：
M. Araki;M. Yamao;M. Tsudzuki
在胚胎早期发育过程中，神经视网膜和视网膜色素上皮（RPE）脱离视神经囊泡。它们起源于视神经囊泡的不同部分，较远的部分发展为神经视网膜，近的部分发展为RPE。由于远端与表皮接触，近端与间充质组织接触，这两部分会遇到不同的环境信号。本研究以一种独特的鹌鹑突变银（B/B）为实验模型，探讨了RPE与来自神经嵴细胞的间充质组织之间相互作用的意义。银突变被认为影响神经嵴来源的组织，包括表皮黑色素细胞。银突变的纯合子具有异常的眼睛，具有双神经视网膜层，这是RPE异常分化形成新的神经视网膜的结果。从早期胚胎眼（过程开始前）取出视网膜色素上皮，并进行培养，观察其是否表达神经视网膜细胞的任何表型特征。将B/B突变体的RPE与周围的间充质组织一起培养，分化出表达无突细胞、锥细胞或杆状细胞标记的神经视网膜细胞。B/B突变体分离的RPE单独培养时，获得了色素沉着，未表现出任何神经视网膜细胞的性质特征。野生型鹌鹑的RPE总是向色素上皮细胞分化。在酸性成纤维细胞生长因子（aFGF）或碱性纤维细胞生长因子（bFGF）存在的情况下，B/B突变体的RPE在没有间充质组织的情况下分化为神经视网膜细胞，而野生型胚胎的RPE仅在aFGF或bFGF存在10-40倍的情况下分化为神经视网膜细胞。这些观察结果表明，负责B/B突变的基因在RPE以及在神经嵴细胞分化中起作用的细胞中表达。他们进一步提出，神经视网膜和RPE的发育是由一些可溶性因子调控的，这些因子来源于或定位于周围的胚胎间质和其他眼部组织，FGF可能是可能的候选者之一。
The neural retina and retinal pigment epithelium (RPE) diverge from the optic vesicle during early embryonic development. They originate from different portions of the optic vesicle, the more distal part developing as the neural retina and the proximal part as RPE. As the distal part appears to make contact with the epidermis and the proximal part faces mesenchymal tissues, these two portions would encounter different environmental signals. In the present study, an attempt has been made to investigate the significance of interactions between the RPE and mesenchymal tissues that derive from neural crest cells, using a unique quail mutant silver (B/B) as the experimental model. The silver mutation is considered to affect neural crest‐derived tissues, including the epidermal melanocytes. The homozygotes of the silver mutation have abnormal eyes, with double neural retinal layers, as a result of aberrant differentation of RPE to form a new neural retina. Retinal pigment epithelium was removed from early embryonic eyes (before the process began) and cultured to see whether it expressed any phenotype characteristic of neural retinal cells. When RPE of the B/B mutant was cultured with surrounding mesenchymal tissue, neural retinal cells were differentiated that expressed markers of amacrine, cone or rod cells. When isolated RPE of the B/B mutant was cultured alone, it acquired pigmentation and did not show any property characteristic of neural retinal cells. The RPE of wild type quail always differentiated to pigment epithelial cells. In the presence of either acidic fibroblast growth factor (aFGF) or basic FGF (bFGF), the RPE of the B/B mutant differentiated to neural retinal cells in the absence of mesenchymal tissue, but the RPE of wild type embryos only did so in the presence of 10–40 times as much aFGF or bFGF. These observations indicate that genes responsible for the B/B mutation are expressed in the RPE as well as in those cells that have a role in the differentiation of neural crest cells. They further suggest that development of the neural retina and RPE is regulated by some soluble factor(s) that is derived from or localized in the surrounding embryonic mesenchyme and other ocular tissues, and that FGF may be among possible candidates.
影响因子：
4.6
作者：
François Guillemot;Constance L. Cepko
通讯作者：
François Guillemot;Constance L. Cepko