Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice
Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice
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DOI：
10.1073/pnas.0508491103
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发表时间：
2006-02-07
影响因子：
11.1
通讯作者：
Ma, MH
中科院分区：
文献类型：
--
作者：
Grosmaitre, X;Vassalli, A;Ma, MH
哺乳动物嗅球中的肾小球接受来自表达相同气味受体（OR）的嗅觉感觉神经元（OSN）的轴突输入。肾小球通常被认为是代表嗅觉编码的功能单位，但没有关于表达相同内源性OR的OSN的电生理特性的数据。在这里，使用膜片钳记录在一个完整的上皮制备，我们直接测量的转导电流和受体电位从小鼠OSN的树突状旋钮表达的气味受体MOR23沿着的绿色荧光蛋白。所有检测的53个细胞都对Lyral有反应，Lyral是MOR23的一种已知配体。在响应动力学方面存在着深刻的差异，特别是在失活阶段。这些细胞对lyral非常敏感，有些细胞对10 nM就有反应。动态范围出乎意料地宽，单个细胞中的阈值和饱和度通常覆盖三个对数单位的里拉醛浓度。这种细胞异质性的潜在原因和生物学意义进行了讨论。膜片钳记录从OSNs，表达一个定义的OR提供了一个强大的方法来调查的感觉输入到个人肾小球。
A glomerulus in the mammalian olfactory bulb receives axonal inputs from olfactory sensory neurons (OSNs) that express the same odorant receptor (OR). Glomeruli are generally thought to represent functional units of olfactory coding, but there are no data on the electrophysiological properties of OSNs that express the same endogenous OR. Here, using patch clamp recordings in an intact epithelial preparation, we directly measured the transduction currents and receptor potentials from the dendritic knobs of mouse OSNs that express the odorant receptor MOR23 along with the green fluorescent protein. All of the 53 cells examined responded to lyral, a known ligand for MOR23. There were profound differences in response kinetics, particularly in the deactivation phase. The cells were very sensitive to lyral, with some cells responding to as little as 10 nM. The dynamic range was unexpectedly broad, with threshold and saturation in individual cells often covering three log units of lyral concentration. The potential causes and biological significance of this cellular heterogeneity are discussed. Patch clamp recording from OSNs that express a defined OR provides a powerful approach to investigate the sensory inputs to individual glomeruli.