Developmental effects in Japanese medaka (Oryzias latipes) exposed to nonylphenol ethoxylates and their degradation products
Developmental effects in Japanese medaka (Oryzias latipes) exposed to nonylphenol ethoxylates and their degradation products
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DOI：
10.1016/j.chemosphere.2005.02.100
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发表时间：
2006-03-01
期刊：
影响因子：
8.8
通讯作者：
Metcalfe, C
中科院分区：
文献类型：
--
作者：
Balch, G;Metcalfe, C
采用日本青鳉（Oryzias latipes）体内试验，研究了壬基酚（NP）、壬基酚聚氧乙烯醚混合物（NP 1 EO、NP 4 EO、NP 9 EO）和壬基酚聚氧乙烯羧酸酯（NP 1 EC）等5种烷基酚类化合物对鱼类的内分泌调节作用。监测孵化后1天至100天暴露于测试材料的青鳉鱼的性别比例和第二性征的改变以及性腺间性的发育（即，testis-ova）。用100 μ g l（-1）NP（测量值为29 μ g l（-1））处理后，80%以上的暴露雄性出现性腺间性，40%以上的暴露鱼类出现混合第二性征，100%雄性的臀鳍乳头发育受到抑制。30 μ g l（-1）NP（测量值为8.7 μ g l（-1））处理仅在22只暴露的雄性中的一只中诱导性腺间性，并在约20%的暴露鱼中诱导混合第二性征。在最高试验浓度300 μ g l（-1）（测量值为105 μ g l（-1））的NP I EO处理中，也观察到具有混合第二性征和乳头状突起发育抑制的鱼类发生率升高。在使用剩余供试混合物的处理中，没有证据表明存在混合第二性征或性腺间性。这项研究证实，NP是一种雌激素化合物，可以影响性腺发育的鱼类长期暴露在浓度范围内的10 μ g l（-1）。NP 1 EO在比壬基酚的最低观察效应浓度高一个数量级的浓度下具有极弱的雌激素性。(c)2005爱思唯尔有限公司保留所有权利。
The endocrine modulating potency of five alkylphenol compounds to fish, including nonylphenol (NP), three nonylphenol ethoxylate mixtures (NP1EO, NP4EO, NP9EO) and one nonylphenol ethoxycarboxylate (NP1EC) was assessed using in vivo tests conducted with Japanese medaka (Oryzias latipes). Medaka exposed to test materials from I day to 100 days post-hatch were monitored for alterations to sex ratios and secondary sex characteristics and development of gonadal intersex (i.e., testis-ova). The treatment with 100 mu g l(-1) NP (measured 29 mu g l(-1)) induced gonadal intersex in over 80% of exposed males, mixed secondary sex characteristics in over 40% of exposed fish and suppression of the development of papillae on the anal fin of 100% of males. The 30 mu g l(-1) NP (measured 8.7 mu g l(-1)) treatment induced gonadal intersex in only one of the 22 exposed males and mixed secondary sex characteristics in approximately 20% of the exposed fish. An elevated incidence of fish with mixed secondary sex characteristics and suppression of papillae development was also observed in the treatment with NP I EO at the highest test concentration of 300 mu g l(-1) (measured 105 mu g l(-1)). There was no evidence of mixed secondary sex characteristics or gonadal intersex in treatments with the remaining test mixtures. This study confirms that NP is an estrogenic compound that could affect gonadal development in fish chronically exposed to concentrations in the range of 10 mu g l(-1). NP1EO is very weakly estrogenic at concentrations that are an order of magnitude higher than the lowest observed effect concentration for nonylphenol. (c) 2005 Elsevier Ltd. All rights reserved.