Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China
Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China
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DOI：
10.1016/j.virusres.2004.05.011
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发表时间：
2004-11-01
期刊：
影响因子：
5
通讯作者：
van Dijk, AA
中科院分区：
文献类型：
--
作者：
Yang, H;Makeyev, E;van Dijk, AA
中国发生的一起成人腹泻疫情是由一种被称为ADRV-N的新型轮状病毒引起的，这种病毒对针对A、B或C组轮状病毒的抗血清不起反应。论文)。[19][1998]。ADRV-N可以在细胞培养物中繁殖[中华微生物学报[Natl]；中华医学杂志，82 (2002)14 [j]。我们给出了ADRV-N基因组第5和6段的完整序列，以及基因组第7段的完整ORF序列。推断出的氨基酸序列表明，这些片段分别编码NSPI、VP6和NSP3。这三个ADRV-N基因组片段具有独特的-ACCCC-3'端序列。第5段和第6段的5′- gg末端与其他轮状病毒相同。ADRV-N的VP6和NSP3与它们最接近的同源序列B组IDIR的氨基酸相似性分别为37%和35%。ADRV-N NSPI具有一个双链RNA结合基序（DSRM）和一个推定的自蛋白水解裂解基序，位于DSRM上游。与B组轮状病毒的同源蛋白相比，推测的ADRV-N NSP3具有截断的c端。所有现有数据表明，ADRV-N与已知的轮状病毒有很大不同，并强烈表明ADRV-N是感染人类的新轮状病毒组中第一个被确认的成员。(C) 2004 Elsevier B.V.版权所有
A diarrhoeal outbreak among adults in China was caused by a new rotavirus, termed ADRV-N, that does not react with antisera directed against group A, B or C rotaviruses [Zhonghua Liu Xing Bing Xue Za Zhi (Chin. Epidemiol.) 19 (1998) 336]. ADRV-N can be propagated in cell cultures [Zhonghua Yi Xue Za Zhi (Natl. Med. J. China) 82 (2002) 14]. We present the complete sequences for ADRV-N genome segments 5 and 6, and a full ORF sequence of genome segment 7. The deduced amino acid sequences suggest that these segments encode NSPI, VP6 and NSP3, respectively. These three ADRV-N genome segments have a unique -ACCCC-3' terminal sequence. The 5'-GGterminus of segments 5 and 6 is the same as that of other rotaviruses. The amino acid similarity between VP6 and NSP3 of ADRV-N and the cognate sequences of their closest counterpart, group B IDIR, was 37 and 35%, respectively. The ADRV-N NSPI has a double-stranded RNA binding motif (DSRM) and a putative autoproteolytic cleavage motif upstream from the DSRM. The putative ADRV-N NSP3 has a truncated C-terminus compared to the cognate protein of group B rotaviruses. All the available data demonstrate that ADRV-N differs significantly from the known rotaviruses and strongly suggest that ADRV-N is the first recognized member of a new group of rotaviruses infecting humans. (C) 2004 Elsevier B.V. All rights reserved.