Isolation and characterization of human placental chorionic villar extracellular matrix.
Isolation and characterization of human placental chorionic villar extracellular matrix.
复制标题
人胎盘绒毛膜绒毛细胞外基质的分离和表征。
DOI：
10.1002/jss.400120405
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
K. Brendel
中科院分区：
文献类型：
--
作者：
M. Ohno;R. Nagle;E. Meezan;K. Brendel
用Triton X-100和脱氧胆酸钠对末端绒毛片段进行萃取，制备了无细胞的人胎盘绒毛细胞外基质。通过扫描和透射电镜观察，分离的人胎盘细胞外基质保留了完整但塌陷的组织结构。它保持完整，在很大程度上是因为存在连续的绒毛基底膜和相关的间质胶原纤维和分散的纤维蛋白斑块。分离的人胎盘细胞外基质的染色特征和化学成分与报道的几种组织的基底膜相似，表明该制剂中存在胶原样蛋白和糖蛋白成分。尿素- sds -巯基乙醇提取物凝胶电泳表明，该基质由多种不同分子量的多肽组分组成，其中一些组分通过二硫键结合成高分子量的配合物。
The cell-free extracellular matrix of human placental chorionic villi has been prepared by a procedure employing extraction of the terminal villar fragments with the detergents Triton X-100 and sodium deoxycholate. The isolated human placental extracellular matrix retains an intact, but collapsed, histoarchitecture, as observed by scanning and transmission electron microscopy. It remains intact, in large part because of the presence of continuous sheets of villar basement membranes and associated interstitial collagen fibers and scattered patches of fibrin. The staining charcteristics and chemical composition of the isolated human placental extracellular matrlix are similar to those reported for basement membranes in several tissues and indicate the presence of collagen-like and glycoprotein components in this preparation. Gel electrophoresis of urea-SDS-mercaptoethanol extracts of the matrix showed that it consists of several polypeptide components of various molecuar weights, some of which are associated into high molecular weight complexes by disulfide bonds.