Alfalfa mosaic virus RNAs serve as cap donors for tomato spotted wilt virus transcription during coinfection of Nicotiana benthamiana
Alfalfa mosaic virus RNAs serve as cap donors for tomato spotted wilt virus transcription during coinfection of Nicotiana benthamiana
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DOI：
10.1128/jvi.73.6.5172-5175.1999
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发表时间：
1999-06-01
影响因子：
5.4
通讯作者：
Goldbach, R
中科院分区：
文献类型：
--
作者：
Duijsings, D;Kormelink, R;Goldbach, R
番茄斑萎病毒（TSWV）在混合侵染本氏烟草（Nicotiana benthamiana）的过程中，利用苜蓿花叶病毒（AMV）RNA作为帽供体。通过使用巢式逆转录-PCR，可以克隆和测序具有来自所有四种AMV RNA的加帽前导序列的TSWV N和NSs mRNA。假定的TSWV核酸内切酶参与的序列特异性进行了讨论。
Tomato spotted wilt virus (TSWV) was shown to use alfalfa mosaic virus (AMV) RNAs as cap donors in vivo during a mixed infection in Nicotiana benthamiana. By use of nested reverse transcription-PCR, TSWV N and NSs mRNAs provided with capped leader sequences derived from all four AMV RNAs could be cloned and sequenced. The sequence specificity of the putative TSWV endonuclease involved is discussed.