Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes.
Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes.
复制标题
定量回收龈下螺旋体可培养物种的三种分散程序的比较。
DOI：
10.1128/jcm.25.11.2230-2232.1987
复制
发表时间：
1987
影响因子：
9.4
通讯作者：
Loesche,WJ
中科院分区：
文献类型：
--
作者：
Salvador,SL;Syed,SA;Loesche,WJ
螺旋体通常是显微镜下观察到的与牙周炎相关的牙齿部位牙龈下斑块中主要的微生物，但这些微生物很少通过培养方法分离出来，可能是因为培养基不支持它们的生长和/或因为这些脆弱的微生物被用于分散斑块样本的各种程序所破坏。在本研究中，比较了三种分散方法，超声、机械混合和均质化，以便在支持这些物种生长的培养基上从菌斑样本中分离出齿状密螺旋体、文氏密螺旋体、索氏密螺旋体和果胶状密螺旋体。选择螺旋体平均占显微镜计数50%的菌斑样本。用Tekmar均质机分散斑块时，螺旋体的活菌回收率最高，超声处理时最低。最高的回收率平均只占总可栽培数量的1%左右，这表明，要么是受追捧的物种是该植物群的次要成员，要么是分散过程仍然过于苛刻。共有91%的分离株为齿形蜱，5%为索氏蜱，4%为迄今未描述的种。
Spirochetes are usually the predominant organisms observed microscopically in subgingival plaques removed from tooth sites associated with periodontitis, but these organisms are rarely isolated by cultural means, presumably because the media do not support their growth and/or because these fragile organisms are disrupted by the various procedures used to disperse plaque samples. In the present investigation, three dispersal procedures, sonification, mechanical mixing, and homogenization, were compared for their ability to permit the isolation of Treponema denticola, Treponema vincentii, Treponema socranskii, and Treponema pectinovorum from plaque samples on media that support the growth of these species. Plaque samples in which the spirochetes averaged 50% of the microscopic count were chosen. The highest viable recoveries of spirochetes were observed when the plaques were dispersed with a Tekmar homogenizer, and the lowest occurred with sonification. The highest recoveries averaged only about 1% of the total cultivable counts, indicating either that the sought-after species were minor members of the flora or that the dispersal procedures were still too harsh. A total of 91% of the isolates were T. denticola, 5% were T. socranskii, and 4% were a heretofore-undescribed species.