Gene expression patterns in blood leukocytes discriminate patients with acute infections
Gene expression patterns in blood leukocytes discriminate patients with acute infections
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DOI：
10.1182/blood-2006-02-002477
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发表时间：
2007-03-01
期刊：
影响因子：
20.3
通讯作者：
Chaussabel, Damien
中科院分区：
文献类型：
--
作者：
Ramilo, Octavio;Allman, Windy;Chaussabel, Damien
每种感染因子代表病原体相关分子模式的独特组合，这些模式与免疫细胞上表达的特定模式识别受体相互作用。因此，我们推测不同感染个体的血液免疫细胞可能具有歧视性转录特征。获得了甲型流感病毒、革兰氏阴性（大肠杆菌）或革兰氏阳性（金黄色葡萄球菌和肺炎链球菌）引起的急性感染患儿131份外周血样本的基因表达谱。鉴定出35个最能区分甲型流感病毒感染患者与大肠杆菌或肺炎链球菌感染患者的基因。这些基因对甲型流感、大肠杆菌或肺炎链球菌感染的一组独立患者的分类准确率为95%（37个样本中的35个）。一种不同的特征区分大肠杆菌和金黄色葡萄球菌感染的准确率为85%（40分之34）。此外，在不同病因的呼吸道感染患者中观察到不同的基因表达模式。因此，对患者外周血白细胞的微阵列分析可能有助于传染病的鉴别诊断。
Each infectious agent represents a unique combination of pathogen-associated molecular patterns that interact with specific pattern-recognition receptors expressed on immune cells. Therefore, we surmised that the blood immune cells of individuals with different infections might bear discriminative transcriptional signatures. Gene expression profiles were obtained for 131 peripheral blood samples from pediatric patients with acute infections caused by influenza A virus, Gram-negative (Escherichia coli) or Gram-positive (Staphylococcus aureus and Streptococcus pneumoniae) bacteria. Thirty-five genes were identified that best discriminate patients with influenza A virus infection from patients with either E coli or S pneumoniae infection. These genes classified with 95% accuracy (35 of 37 samples) an independent set of patients with either influenza A, E coli, or S pneumoniae infection. A different signature discriminated patients with E coli versus S aureus infections with 85% accuracy (34 of 40). Furthermore, distinctive gene expression patterns were observed in patients presenting with respiratory infections of different etiologies. Thus, microarray analyses of patient peripheral blood leukocytes might assist in the differential diagnosis of infectious diseases.