In Situ Electrodeposited Synthesis of Electrochemiluminescent Ag Nanoclusters as Signal Probe for Ultrasensitive Detection of Cyclin-D1 from Cancer Cells.
In Situ Electrodeposited Synthesis of Electrochemiluminescent Ag Nanoclusters as Signal Probe for Ultrasensitive Detection of Cyclin-D1 from Cancer Cells.
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DOI：
10.1021/acs.analchem.7b01154
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发表时间：
2017-06
影响因子：
7.4
通讯作者：
Ying Zhou;Maoxin Chen;Ying Zhuo;Y. Chai;Wenju Xu;R. Yuan
中科院分区：
文献类型：
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作者：
Ying Zhou;Maoxin Chen;Ying Zhuo;Y. Chai;Wenju Xu;R. Yuan
金属纳米团簇（NCs）作为一种新型电化学发光（ECL）纳米材料引起了人们的广泛关注，但由于发光效率相对较低且制备工艺复杂，其应用受到限制。在此，利用原位电生银纳米簇（AgNCs）作为ECL发射体和Fe3O4-CeO2纳米复合材料作为共反应加速剂，设计了一种用于检测Cyclin-D1（CCND1）的超灵敏ECL生物传感器。利用Fe3O4-CeO2加速S2O82-还原生成强氧化性中间体自由基SO4·-，可以显着提高AgNCs在电极上的ECL发光效率。因此，CCND1 检测方法实现了出色的灵敏度，线性范围为 50 fg/mL 至 50 ng/mL，检测限低至 28 fg/mL。令人印象深刻的是，由于 CCND1 的过度表达与 MCF-7 人乳腺癌细胞的生长和转移有关，因此成功研究了中药槐花对 MCF-7 细胞的功效。总的来说，所提出的策略为抗癌药物筛选提供了有效的方法，并扩大了金属NC在超灵敏生物检测中的应用。
Metal nanoclusters (NCs) as a new type of electrochemiluminescence (ECL) nanomaterials have attracted great attention, but their applications are limited due to relatively low luminescent efficiency and a complex preparation process. Herein, an ultrasensitive ECL biosensor for the detection of Cyclin-D1 (CCND1) was designed by utilizing in situ electrogenerated silver nanoclusters (AgNCs) as ECL emitters and Fe3O4-CeO2 nanocomposites as a coreaction accelerator. The ECL luminous efficiency of AgNCs on the electrode could be significantly enhanced with the use of the Fe3O4-CeO2 for accelerating the reduction of S2O82- to generate the strong oxidizing intermediate radical SO4•-. As a result, the assay for CCND1 detection achieved excellent sensitivity with a linear range from 50 fg/mL to 50 ng/mL and limit of detection down to 28 fg/mL. Impressively, the efficiency of Traditional Chinese Medicines (TCM), sophorae, toward MCF-7 cells was successfully investigated due to the overexpression of CCND1 in relation to the growth and metastasis of MCF-7 human breast cancer cells. In general, the proposed strategy provided an effective method for anticancer drug screening and expanded the application of metal NCs in ultrasensitive biodetection.