Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity
Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity
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DOI：
10.1194/jlr.p005298
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发表时间：
2010-08-01
影响因子：
6.5
通讯作者：
Harper, Mary-Ellen
中科院分区：
文献类型：
--
作者：
Gerrits, Martin F.;Ghosh, Sujoy;Harper, Mary-Ellen
在能量过剩和能量不足的条件下，体重增加和减少的个体间差异是公认的，但了解甚少的现象。我们记录了在一个强化监督的临床减肥计划中的减肥变异性，并评估了与900千卡方案的可变反应相关的骨骼肌基因表达和表型特征。健康、饮食依从、肥胖饮食敏感（ODS）和饮食抵抗（ODR）受试者的配对定义为体重减轻率最高和最低的五分位数。体力活动的能量消耗是最小的和可比的。在程序完成和体重稳定后，获得骨骼肌活检。股直肌和股外侧肌的基因表达分析表明，与ODR相比，ODS中参与氧化磷酸化和葡萄糖和脂肪酸代谢的基因和基因组上调。在股外侧肌中，ODS中氧化（I型）纤维的比例高于ODR妇女和瘦对照组，ODS中纤维肥大与ODR妇女和瘦对照组相比，所有肥胖者中氧化和氧化糖酵解纤维中琥珀酸脱氢酶的比例低于瘦对照组。肌内脂质含量一般较高，肥胖与瘦，特别是较高的ODS与瘦的妇女。总而言之，我们的研究结果证明了肌肉基因表达和纤维成分与临床减肥成功相关的差异。Gerrits，M. F.、S. Ghosh，N.卡瓦斯拉尔湾，B。希尔A.图，E。L.塞弗特，B。Beauchamp，S.作者声明：R.登特河麦克弗森和M-E。哈珀。饮食敏感型肥胖与饮食抵抗型肥胖的骨骼肌纤维特征和基因表达差异。J. Lipid Res. 51：23942404.
Inter-individual variability in weight gain and loss under energy surfeit and deficit conditions, respectively, are well recognized but poorly understood phenomena. We documented weight loss variability in an intensively supervised clinical weight loss program and assessed skeletal muscle gene expression and phenotypic characteristics related to variable response to a 900 kcal regimen. Matched pairs of healthy, diet-compliant, obese diet-sensitive (ODS) and diet-resistant (ODR) subjects were defined as those in the highest and lowest quintiles for weight loss rate. Physical activity energy expenditure was minimal and comparable. Following program completion and weight stabilization, skeletal muscle biopsies were obtained. Gene expression analysis of rectus femoris and vastus lateralis indicated upregulation of genes and gene sets involved in oxidative phosphorylation and glucose and fatty acid metabolism in ODS compared with ODR. In vastus lateralis, there was a higher proportion of oxidative (type I) fibers in ODS compared with ODR women and lean controls, fiber hypertrophy in ODS compared with ODR women and lean controls, and lower succinate dehydrogenase in oxidative and oxidative-glycolytic fibers in all obese compared with lean subjects. Intramuscular lipid content was generally higher in obese versus lean, and specifically higher in ODS vs. lean women. Altogether, our findings demonstrate differences in muscle gene expression and fiber composition related to clinical weight loss success.-Gerrits, M. F., S. Ghosh, N. Kavaslar, B. Hill, A. Tour, E. L. Seifert, B. Beauchamp, S. Gorman, J. Stuart, R. Dent, R. McPherson, and M-E. Harper. Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity. J. Lipid Res. 51: 23942404.