Insecticide resistance of Triatoma infestans (Hemiptera, Reduviidae) vector of Chagas disease in Bolivia
Insecticide resistance of Triatoma infestans (Hemiptera, Reduviidae) vector of Chagas disease in Bolivia
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DOI：
10.1111/j.1365-3156.2010.02573.x
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发表时间：
2010-09-01
影响因子：
3.3
通讯作者：
Chavez, Tamara
中科院分区：
文献类型：
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作者：
Lardeux, Frederic;Depickere, Stephanie;Chavez, Tamara
目的了解玻利维亚致病三角毛虫对溴氰菊酯(拟除虫菊酯)、马拉硫磷(有机磷)和杀菌威(氨基甲酸酯)的抗药性状况。用0.2亩L杀虫剂丙酮溶液对F1代若虫N1进行局部施药，获得了定量反应数据。对大多数人群的剂量-死亡率关系和抗性比(RR)进行了分析。每种杀虫剂都与敏感的参考菌株建立了区分浓度，并用于其他田间种群。结果溴氰菊酯、敌敌畏和马拉硫磷的有效成分平均浓度分别为5、70和120 ng/头。溴氰菊酯接触1h的诊断浓度为0.30%。在人类住宅中采样的所有种群对溴氰菊酯表现出显著的抗性水平，从6到491，并且在不同地区有所不同。当使用增效剂胡椒基丁醚(PBO)时，抗性种群不能完全恢复对溴氰菊酯的敏感性。没有一个样本种群对苯敌畏(全部RR(50)和1.8)或马拉硫磷(全部RR(50)和2.2)表现出显著的抗药性。结论在玻利维亚，大多数家养的致病线虫对溴氰菊酯具有抗药性。由于杀虫剂媒介控制是唯一的选择压力，抗性很可能源于杀虫剂媒介。从拟除虫菊酯转向有机磷或氨基甲酸酯可能是控制该媒介的短期解决方案，但从长远来看，还应考虑其他替代的综合防治策略。
P>ObjectiveTo define the insecticide resistance status of Triatoma infestans to deltamethrin (pyrethroid), malathion (organophosphate) and bendiocarb (carbamate) in Bolivia.MethodsFifty populations of T. infestans were sampled in Bolivian human dwellings. Quantal response data were obtained by topical applications of 0.2 mu l of insecticide-acetone solutions on nymphs N1 of the F1 generations. For most populations, dose-mortality relationships and resistance ratios (RR) were analysed. Discriminating concentrations were established for each insecticide with a susceptible reference strain and used on the other field populations. A tarsal-contact diagnostic test using insecticide impregnated papers was designed to rapidly identify deltamethrin-resistant populations in the field.ResultsDiscriminating concentrations for topical applications were 5, 70 and 120 ng active ingredient per insect for deltamethrin, bendiocarb and malathion, respectively. The diagnostic concentration for deltamethrin was 0.30% for the 1-h exposure by tarsal contact. All populations sampled in human dwellings exhibited significant levels of resistance to deltamethrin, from 6 to 491 and varied among regions. Resistant populations did not recover complete susceptibility to deltamethrin when the synergist piperonyl butoxide (PBO) was used. None of the sampled populations exhibited significant resistance to bendiocarb (all RR(50) < 1.8) or malathion (all RR(50) < 2.2).ConclusionIn Bolivia, most 'domestic'T. infestans populations are resistant to deltamethrin. Because insecticide vector control is the only selection pressure, resistance likely originates from it. Switching from pyrethroids to organophosphates or carbamates could be a short-term solution to control this vector, but other alternative integrated control strategies should also be considered in the long term.