A Novel Purification Method for Multipotential Skeletal Stem Cells
A Novel Purification Method for Multipotential Skeletal Stem Cells
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DOI：
10.1002/jcb.22262
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发表时间：
2009-10-01
影响因子：
4
通讯作者：
Aubin, Jane E.
中科院分区：
文献类型：
--
作者：
Itoh, Shousaku;Aubin, Jane E.
至少。骨髓基质细胞群中的一些细胞具有多能性（即在体外分化为成骨细胞、软骨细胞和脂肪细胞），因此被称为骨骼干细胞（SSC）或间充质干细胞（MSC）等。最近，基质细胞亚群，特别是成骨细胞或其祖细胞，已被确定为造血干细胞（HSC）生态位的明确调节成分。因此，开发不仅纯化 SSC，而且纯化包含 HSC 生态位的细胞的方法也很有意义。在这里，我们报告了一种纯化新型骨髓来源群体的方法，该群体具有高频率的骨祖细胞和高表达水平的成骨细胞分化标记物（高度纯化的骨祖细胞；（HipOPs））以及HSC的骨生态位标记物。体内移植实验表明，供体 HipOP 不仅可以分化为成骨细胞、骨细胞和血窦周围的细胞，还可以分化为造血细胞。因此，HipOP 代表了一个新的群体，即骨和骨髓微环境的同步重建。 J.细胞。生物化学。 108: 368-377, 2009。(C) 2009 Wiley-Liss, Inc.
At least. some cells within bone marrow stromal populations are multipotential (i.e., differentiate in vitro into osteoblasts, chondrocytes, and adipocytes) and thus designated skeletal stem cells (SSCs) or mesenchymal stem cells (MSCs) amongst other names. Recently, a subpopulation of stromal cells, notably osteoblasts or their progenitors, has been identified as a definitive regulatory component of the hematopoietic stem cell (HSC) niche. Thus, the development of methods for purifying not only SSCs but cells comprising the HSC niche is of interest. Here, we report a method for purifying a novel bone marrow-derived population with a high frequency of osteoprogenitors and high expression levels of osteoblast differentiation markers (highly purified osteoprogenitors; (HipOPs)) as well as markers of the bone niche for HSCs. In vivo transplantation experiments demonstrated that donor HipOPs differentiated into not only osteoblasts, osteocytes and cells around sinusoids hut also hematopoietic cells. Thus, HipOPs represent a novel population I-or simultaneous reconstruction of bone and bone marrow microenvironments. J. Cell. Biochem. 108: 368-377, 2009. (C) 2009 Wiley-Liss, Inc.