Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium
Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium
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DOI：
10.1111/j.1600-0714.2010.00981.x
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发表时间：
2011-05-01
影响因子：
3.3
通讯作者：
Williams, David W.
中科院分区：
文献类型：
--
作者：
Silva, Sonia;Henriques, Mariana;Williams, David W.
背景：白色念珠菌被认为是念珠菌病的主要致病菌。然而，光滑念珠菌已成为口腔粘膜的一种重要病原体，既可单独感染，也可混合感染。白色念珠菌与C.白色念珠菌，很少有人知道的作用。口腔感染中的glabrata本研究的目的是检查口腔上皮中涉及C的单一和混合种感染。方法：用C. glabrata感染重组人口腔上皮细胞（RHOE），建立其侵袭和损伤组织的能力。glabrata，或与C. glabrata和C.白色念珠菌。这两个物种入侵组织的能力进行了检查，使用物种特异性肽核酸（PNA）探针杂交和共聚焦激光扫描显微镜。通过测量乳酸脱氢酶（LDH）activity.Results：光滑念珠菌菌株能够定殖的RHOE，在一个应变依赖性的方式进行评估上皮细胞的损伤。光滑念珠菌单次感染12 h后，通常显示RHOE无侵袭，这与C.白色念珠菌。混合感染表明，C.白色念珠菌增强了念珠菌的侵袭力。glabrata，并导致增加LDH释放的RHOE，这掩盖了所观察到的组织损伤。结论：所获得的结果表明，增强的侵袭和增加的组织损伤所造成的混合C。glabrata和C.白色念珠菌感染具有重要的临床意义，并强调需要确定涉及口腔念珠菌病的念珠菌属。
Background:Candida albicans is regarded as the leading of candidosis. However, Candida glabrata has emerged as an important pathogen of oral mucosa, occurring both singly or in mixed species infections, often with C. albicans. Compared with C. albicans, little is known about the role of C. glabrata in oral infection. The aim of this study was to examine single and mixed species infection of oral epithelium involving C. glabrata and establish its ability to invade and damage tissue.Methods:A reconstituted human oral epithelium (RHOE) was infected only with C. glabrata, or simultaneously with C. glabrata and C. albicans. The ability of both species to invade the tissue was examined using species specific peptide nucleic acid (PNA) probe hybridization and confocal laser scanning microscopy. Epithelial damage was assessed by measuring lactate dehydrogenase (LDH) activity.Results:Candida glabrata strains were able to colonize the RHOE, in a strain dependent manner. Candida glabrata single infection after 12 h, generally revealed no invasion of the RHOE, which contrasted with extensive tissue invasion demonstrated by C. albicans. Mixed infection showed that C. albicans enhanced the invasiveness of C. glabrata, and led to increased LDH release by the RHOE, which paralleled the observed histological damage.Conclusions:The results obtained demonstrating enhanced invasion and increased tissue damage caused by mixed C. glabrata and C. albicans infections have important clinical significance and highlight the need to identify Candida species involved in oral candidosis.