The fates of chicken nuclear lamin proteins during mitosis: evidence for a reversible redistribution of lamin B2 between inner nuclear membrane and elements of the endoplasmic reticulum
The fates of chicken nuclear lamin proteins during mitosis: evidence for a reversible redistribution of lamin B2 between inner nuclear membrane and elements of the endoplasmic reticulum
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鸡核核纤层蛋白在有丝分裂过程中的命运：核纤层蛋白 B2 在内核膜和内质网元件之间可逆重新分布的证据
DOI：
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发表时间：
1988
影响因子：
7.8
通讯作者：
Erich A. Nigg
中科院分区：
文献类型：
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作者：
R. Stick;Brigitte Angres;Christian E Lehner;Erich A. Nigg
在鸡中，已经描述了三种结构不同的核纤层蛋白。根据它们在二维凝胶上的迁移，这些蛋白质被指定为核纤层蛋白 A、B1 和 B2。为了研究鸡核纤层蛋白与其哺乳动物对应物之间的功能关系，我们在这里检查了个体鸡核纤层蛋白在有丝分裂过程中的状态。当前提出哺乳动物核纤层蛋白亚型功能特化的模型实际上主要基于这样的观察：在有丝分裂期间，哺乳动物核纤层蛋白B仍然与膜囊泡相关，而核纤层蛋白A和C变得可自由溶解。细胞分级实验与免疫印迹相结合表明，在有丝分裂过程中，鸡核纤层蛋白 B1 和 B2 仍与膜结合，而核纤层蛋白 A 以可溶形式存在。对有丝分裂细胞进行的原位免疫电子显微镜也揭示了核纤层蛋白 B2 的膜关联，而核纤层蛋白 A 的分布是随机的。从这些结果中，我们得出结论，鸡核纤层蛋白 B1 和 B2 在功能上可能类似于哺乳动物核纤层蛋白 B。有趣的是，有丝分裂细胞的免疫标记揭示了核纤层蛋白 B2 与类似于内质网元件的延伸膜池的关联。定量地，我们发现中期细胞中存在的所有大内质网样膜都装饰有核纤层蛋白 B2 特异性抗体。鉴于这些有丝分裂膜的标记低于间期核膜的标记，看来在核膜核膜层蛋白 B2 的有丝分裂分解和重组过程中可能可逆地分布在内核膜和内质网之间。
In chicken, three structurally distinct nuclear lamin proteins have been described. According to their migration on two-dimensional gels, these proteins have been designated as lamins A, B1, and B2. To investigate the functional relationship between chicken lamins and their mammalian counterparts, we have examined here the state of individual chicken lamin proteins during mitosis. Current models proposing functional specializations of mammalian lamin subtypes are in fact largely based on the observation that during mitosis mammalian lamin B remains associated with membrane vesicles, whereas lamins A and C become freely soluble. Cell fractionation experiments combined with immunoblotting show that during mitosis both chicken lamins B1 and B2 remain associated with membranes, whereas lamin A exists in a soluble form. In situ immunoelectron microscopy carried out on mitotic cells also reveals membrane association of lamin B2, whereas the distribution of lamin A is random. From these results we conclude that both chicken lamins B1 and B2 may functionally resemble mammalian lamin B. Interestingly, immunolabeling of mitotic cells revealed an association of lamin B2 with extended membrane cisternae that resembled elements of the endoplasmic reticulum. Quantitatively, we found that all large endoplasmic reticulum-like membranes present in metaphase cells were decorated with lamin B2-specific antibodies. Given that labeling of these mitotic membranes was lower than labeling of interphase nuclear envelopes, it appears likely that during mitotic disassembly and reassembly of the nuclear envelope lamin B2 may reversibly distribute between the inner nuclear membrane and the endoplasmic reticulum.
细胞核：结构、功能和动力学。
影响因子：
16.6
作者：
Newport,JW;Forbes,DJ
通讯作者：
Forbes,DJ