NON-ESTERIFIED FATTY ACIDS IN THE BLOOD OF OBESE AND LEAN SUBJECTS
NON-ESTERIFIED FATTY ACIDS IN THE BLOOD OF OBESE AND LEAN SUBJECTS
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DOI：
10.1093/ajcn/8.5.740
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发表时间：
1960-01-01
影响因子：
7.1
通讯作者：
GORDON, ES
中科院分区：
文献类型：
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作者：
GORDON, ES
这是一个共同的信念，pilysicians和门外汉一样，基本的差异，在能量代谢必须存在之间的肥胖和瘦的人类受试者。尽管生物化学家和生理学家未能确定任何明确的反常现象，但人们仍然相信，肥胖症患者较高的总体热力学效率可以解释他们对能量的明显低需求和对减肥的抵抗力，当他们遭受持续的热量不足时，这种抵抗力就会表现出来。本文的报告涉及与这个问题有关的调查，因为观察到在禁食条件下脂肪动员和利用的模式在胖和瘦的受试者中明显不同。非酯化脂肪酸（NEFA）已通过滴定法测量，该滴定法描述为“静脉血在全天中间隔抽取，使用肝素作为抗凝剂。立即通过离心和分离血浆进行处理，然后用庚烷提取。使用溴百里酚蓝作为指示剂，在氮气流搅拌的两相系统中用稀氢氧化钠进行最终滴定。所有受试者在整个测试过程中均禁食;第一份血液样本于上午8：00采集。在禁食一夜之后，最后一个样本在下午4：00和下午5：00之间抽取，50表明测试时间段代表24小时禁食的最后9小时。安静
IT IS A common belief of pilysicians and laymen alike that basic differences in energy metabolism must exist between obese and thin human subjects. Despite the failure of biochemists and physiologists to identify any definite anotmialy, the belief has persisted that a higher over-all thermodynamic efficiency of obese lersosms could explain their apparent low requirements of energy and the resistance to weight loss which beconmes manifest when they are subjected to a sustained caloric deficit. The report herein deals with investigations bearing upon this problem as a result of the observation that the pattern of fat mobilization and utilization under fasting conditions is demonstrably different in fat and thin subjects.Non-esterified fatty acids (NEFA) have been measured by the titration method described by‘Venous blood is drawn at intervals throughout the day, using heparin as the anticoagulant. It is processed immediately by centrifugation and separation of the plasma, which is then extracted with heptane. Final titration with dilute sodium hydroxide is per-formed in a two-phase system agitated with a stream of nitrogen, using bromthymol blue as an indicator. All subjects are fasted through-out the test; the first blood sample is drawn at 8: 00 AM. after an overnight fast and the last specimen drawn between 4: 00 PM and 5: 00 PM, 50 that the test period represents the last nine hours of a twenty-four hour fast. Quiet