Isolation of Biosurfactant from Lactobacillus sp. and Study of Its Inhibitory Properties Against E. coli Biofilm
Isolation of Biosurfactant from Lactobacillus sp. and Study of Its Inhibitory Properties Against E. coli Biofilm
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DOI：
10.22207/jpam.13.1.44
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发表时间：
2019-03-01
影响因子：
0.8
通讯作者：
George, Sona
中科院分区：
文献类型：
--
作者：
Emmanuel, E. S. Challaraj;Priya, S. Sree;George, Sona
细菌在自然界中无处不在，通过在任何合适的基质上形成生物膜而在所有环境中生存。生物膜可以在环境和人体中藏匿人类感染因子。大肠杆菌是人类胃肠道中最重要的物种，具有在体内和体外形成生物膜的能力。生物表面活性剂在工业和医疗应用的生物技术中发挥着重要作用。表面疏水性通过生物表面活性剂的吸附而改变，从而干扰微生物的结合和解吸过程。益生菌在体内产生的生物表面活性剂可以作为防御武器，对抗泌尿生殖道、胃肠道以及医疗器械中最麻烦的致病菌株。生物表面活性剂比化学表面活性剂显示出有希望的结果，因为它们具有更好的生物降解性并且不污染环境。乳酸菌是一种益生菌，通过产生各种抗菌剂而被认为是潜在的干扰细菌。在目前的研究中，生产生物表面活性剂的乳杆菌属。从自制凝乳样品中分离出来。 TLC结果表明其为脂肽类生物表面活性剂。 FTIR 分析主要显示烯烃和炔基的存在。将生物表面活性剂对大肠杆菌的抗菌活性与不同浓度的化学表面活性剂SDS进行比较，结果表明具有相似的抗菌活性。生物膜抑制测定表明，乳杆菌产生的生物表面活性剂可抑制大肠杆菌的生物膜。
Bacteria are ubiquitous in nature, survive in all environment by forming biofilm on any suitable substrata. Biofilms can harbor human infectious agents in the environment as well in the human body. E. coli is a most important species that is found in the human gastrointestinal tract and has the ability to form biofilm both In vivo and In vitro. Biosurfactants play a significant role in biotechnology for industrial and medical applications. The surface hydrophobicity is modified by the adsorption of biosurfactants, thus interferes in the microbial binding and desorption processes. The production of biosurfactants by probiotic bacteria in vivo acts as a defense weapon against most troublesome pathogenic strains in the urogenital and gastrointestinal tracts and on medical devices. The biosurfactant shows promising results than the chemical surfactant as they are better biodegradable and do not pollute the environment. Lactobacillus is a probiotic bacterium which is recognized as potential interfering bacteria by producing various antimicrobial agents. In the current study, biosurfactant producing Lactobacillus sp. isolated from homemade curd sample. The TLC result indicates that it is a lipopeptide biosurfactant. The FTIR analysis predominantly shows the presence of alkene and alkyne groups. The antimicrobial activity of biosurfactant against E. coli is compared with different concentrations of chemical surfactant SDS and the results indicate a similar antimicrobial activity. The biofilm inhibition assays suggest that the biosurfactant produced by Lactobacillus inhibits the biofilm of E. coli.