Bus, a bushy arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates
Bus, a bushy arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates
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DOI：
10.1105/tpc.13.2.351
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发表时间：
2001-02-01
期刊：
影响因子：
11.6
通讯作者：
Palme, K
中科院分区：
文献类型：
--
作者：
Reintanz, B;Lehnen, M;Palme, K
拟南芥的一个新突变体被命名为bus1-1（浓密），其表现出浓密的表型，叶子起皱，维管化延迟。该表型是由基因 CYP79F1 中的 En-1 插入引起的。推导的蛋白质属于细胞色素P450超家族。因为 CYP79 亚家族的成员被认为能催化氨基酸氧化为醛肟，这是芥子油苷生物合成的第一步，所以我们分析了 CYP79F1 无效突变体 (bus1-1f) 和过表达植物中的芥子油苷水平。源自蛋氨酸的短链芥子油苷在无效突变体中完全缺乏，而在过表达植物中表现出增加的水平，表明CYP79F1使用短链蛋氨酸衍生物作为底物。此外，在bus1-1f突变体中，吲哚-3-基甲基-芥子油苷的浓度以及生长素吲哚-3-乙酸及其前体吲哚-3-乙腈的含量增加。我们的结果首次证明，蛋氨酸衍生的芥子油苷的形成是由 CYP79F1 介导的，并且敲除这种细胞色素 P450 对植物生长和发育具有深远的影响。
A new mutant of Arabidopsis designated bus1-1 (for bushy), which exhibited a bushy phenotype with crinkled leaves and retarded vascularization, was characterized. The phenotype was caused by an En-l insertion in the gene CYP79F1. The deduced protein belongs to the cytochrome P450 superfamily. Because members of the CYP79 subfamily are believed to catalyze the oxidation of amino acids to aldoximes, the initial step in glucosinolate biosynthesis, we analyzed the level of glucosinolates in a CYP79F1 null mutant (bus1-1f) and in an overexpressing plant. Short-chain glucosinolates derived from methionine were completely lacking in the null mutant and showed increased levels in the overexpressing plant, indicating that CYP79F1 uses short-chain methionine derivatives as substrates, In addition, the concentrations of indole-3-ylmethyl-glucosinolate and the content of the auxin indole-3-acetic acid and its precursor indole-3-acetonitrile were increased in the bus1-1f mutant. Our results demonstrate for the first time that the formation of glucosinolates derived from methionine is mediated by CYP79F1 and that knocking out this cytochrome P450 has profound effects on plant growth and development.