A systematic quantification of carbonic anhydrase transcripts in the mouse digestive system
A systematic quantification of carbonic anhydrase transcripts in the mouse digestive system
复制标题
DOI：
10.1186/1471-2199-8-22
复制
发表时间：
2007-03-16
影响因子：
--
通讯作者：
Parkkila, Seppo
中科院分区：
文献类型：
--
作者：
Pan, Pei-wen;Rodriguez, Alejandra;Parkkila, Seppo
背景资料：碳酸酐酶（Carbonic anhydrase，CA）是一种重要的生理酶，参与胃肠道的酸和碳酸氢盐的分泌以及代谢途径，包括尿素生成和尿素生成。基因组数据表明，哺乳动物CA同工酶家族有13个酶活性成员。本研究采用实时荧光定量PCR技术，系统检测了公、母小鼠消化系统8种组织中CA同工酶的mRNA表达水平。结果：所有组织中CA均为Car5b、Car7和Car15，其中Car5b为中等水平表达，Car7和Car15极低水平表达。Car3、Car12、Car13和Car14在8个组织中的7个中被检测到，Car2和Car4在6个组织中被表达。重要的是，与其他CA相比，Car1、Car3和Car13在某些组织中表现出非常高的表达水平，这表明这些低活性同工酶也可能参与CA催化以外的生理过程，并且需要高表达水平才能在体内发挥其功能。在本研究中产生的13种酶活性CA在小鼠胃肠道中的全面mRNA表达谱。这有助于更深入地了解CA同工酶在小鼠消化系统中的分布及其潜在作用。
Background: Carbonic anhydrases (CAs) are physiologically important enzymes which participate in many gastrointestinal processes such as acid and bicarbonate secretion and metabolic pathways including gluconeogenesis and ureagenesis. The genomic data suggests that there are thirteen enzymatically active members of the mammalian CA isozyme family. In the present study, we systematically examined the mRNA expression levels of all known CA isozymes by quantitative real-time PCR in eight tissues of the digestive system of male and female mice.Results: The CAs expressed in all tissues were Car5b, Car7, and Car15, among which Car5b showed moderate and Car7 and Car15 extremely low expression levels. Car3, Car12, Car13, and Car14 were detected in seven out of eight tissues and Car2 and Car4 were expressed in six tissues. Importantly, Car1, Car3, and Car13 showed very high expression levels in certain tissues as compared to the other CAs, suggesting that these low activity isozymes may also participate in physiological processes other than CA catalysis and high expression levels are required to fulfil their functions in the body.Conclusion: A comprehensive mRNA expression profile of the 13 enzymatically active CAs in the murine gastrointestinal tract was produced in the present study. It contributes to a deeper understanding of the distribution of CA isozymes and their potential roles in the mouse digestive system.