Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
Printed covalent glycan array for ligand profiling of diverse glycan binding proteins
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DOI：
10.1073/pnas.0407902101
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发表时间：
2004-12-07
影响因子：
11.1
通讯作者：
Paulson, JC
中科院分区：
文献类型：
--
作者：
Blixt, O;Head, S;Paulson, JC
在这里，我们描述了一种聚糖微阵列，通过使用标准的机器人微阵列打印技术来将胺官能化聚糖偶联到氨基反应性载玻片上。该阵列包含200个合成和天然聚糖序列，代表糖蛋白和糖脂的主要聚糖结构。该阵列具有显着的实用性，用于分析各种聚糖结合蛋白的特异性，包括C型凝集素，单细胞凝集素，半乳糖凝集素，抗碳水化合物抗体，植物和微生物的凝集素，和完整的病毒。
Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.