Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro.
Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro.
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DOI：
10.1530/rep.1.00548
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发表时间：
2005-04
期刊：
影响因子：
3.8
通讯作者：
C. Hidalgo;C. Díez;P. Duque;J. M. Prendes;Aida Rodríguez;F. Goyache;Iván Fernández;N. Facal
中科院分区：
文献类型：
--
作者：
C. Hidalgo;C. Díez;P. Duque;J. M. Prendes;Aida Rodríguez;F. Goyache;Iván Fernández;N. Facal
类维生素a已被证明能提高牛、羊和猪卵母细胞的发育能力。在这项研究中，我们研究了外源性视黄醇是否刺激牛卵母细胞在卵泡内生长和暴露于外源性视黄醇的时间限制。此外，我们还确定了结合视黄醇处理的取卵技术的效率和胚胎移植后的存活能力。在实验1中，分别给小母牛注射视黄醇或对照物，并在第0天（注射前）、第1天和第4天与卵泡液一起分析血液中视黄醇的浓度。血液中的视黄醇在第1天升高，在第4天清除，但在卵泡内视黄醇仍然较高。在实验2中，卵母细胞供体每周注射视黄醇或载体4次，每周两次，共8次恢复周期（从第一次恢复前4天开始），随后是第二个8次不治疗周期。将恢复的卵母细胞在体外受精培养。视黄醇治疗在整个过程中产生了更多的低质量卵母细胞，尽管在周期中测量的视黄醇没有变化。在视黄醇治疗后的前5个疗程中，总卵母细胞、毛囊和囊胚率增加。正如先前从屠宰场卵巢中提取的卵母细胞所显示的，维甲酸刺激了囊胚的发育。在移植到受体后，暴露于视黄醇的卵母细胞的囊胚不能怀孕。本研究证实了视黄醇对奶牛卵泡内卵母细胞的影响，为视黄醇的致畸作用提供了证据。
Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and cultured in vitro. Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.