Incompatible pollen tube growth and protein composition in styles of Japanese pear following high temperature treatments
Incompatible pollen tube growth and protein composition in styles of Japanese pear following high temperature treatments
复制标题
高温处理后日本梨花粉管生长和蛋白质组成不相容
DOI：
10.1007/bf00023053
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发表时间：
1989
期刊：
影响因子：
1.9
通讯作者：
A. Tomita
中科院分区：
文献类型：
--
作者：
S. Hiratsuka;A. Tomita
将自交不亲和的日本梨（Pyrus pyrus）的离体预授粉花柱浸入水中，在45°C蒸馏水中浸泡1.5- 2.5min，可促进自花花粉管的生长，使花柱穿过花粉管到达切端的数量和从切端伸出的花粉管数量增加。这些处理也促进了伸出的花粉管在琼脂培养基上的后续生长。40°C处理不能促进自花花粉管的生长，50°C处理导致花柱坏死。在通过等电聚焦聚丙烯酰胺凝胶电泳分析的花柱蛋白质中，在热处理之后发现带型的几个变化;其中一条带是糖蛋白，随着花柱的自交不亲和性变得更强，糖蛋白在发育中的花柱中数量增加（平冢等人，1986年）。考马斯亮蓝-G250染料对该蛋白质条带的染色较弱。用免疫扩散法检测花柱中的抗原物质，高温处理似乎不会使其变性。
Immersing the excised-prepollinated styles of self-incompatible Japanese pear,Pyrus serotinaRehd., into distilled water at 45°C for 1.5–2.5 min promoted the growth of self-pollen tubes; both the number of styles penetrating the pollen tubes through styles to cut ends and of pollen tubes protruding from cut ends were increased. The subsequent growth of the protruded pollen tubes on agar media was also enhanced by the treatments. Treatments at 40°C did not promote the growth of self-pollen tubes and those at 50°C led the styles to necrosis. In the stylar proteins analyzed by isoelectricfocusing polyacrylamide gel electrophoresis, several changes in banding pattern were found following the heat treatment; one of the bands was a glycoprotein that increases in quantity in developing styles as self-incompatibility of the style becomes stronger (Hiratsuka et al., 1986). This protein band was stained more weakly by Coomassie Brilliant Blue-G250 dye. Antigenic substances in the styles examined by immunodiffusion seemed not to be denatured by high temperature treatments.