Access to RNA Encapsidated in the Nucleocapsid of Vesicular Stomatitis Virus
Access to RNA Encapsidated in the Nucleocapsid of Vesicular Stomatitis Virus
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DOI：
10.1128/jvi.01927-10
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发表时间：
2011-03-01
影响因子：
5.4
通讯作者：
Luo, Ming
中科院分区：
文献类型：
--
作者：
Green, Todd J.;Rowse, Michael;Luo, Ming
负链RNA病毒，例如水泡性口炎病毒（VSV），其基因组RNA在病毒感染的每个阶段都被核衣壳蛋白（N）完全包裹。然而，在病毒转录/复制过程中，核衣壳中的基因组 RNA 必须能够被病毒编码的 RNA 依赖性 RNA 聚合酶访问，才能作为 RNA 合成的模板。通过在大肠杆菌中产生的 VSV 核衣壳和核衣壳样颗粒 (NLP)，我们发现 VSV 核衣壳中的 RNA 可以被 RNase A 去除，这与之前报道的情况相反。 RNA 的去除不会破坏 N 蛋白的组装，从而产生空的衣壳。将多核糖核苷酸重新封装到空 NLP 中，并确定晶体结构。晶体结构揭示了 N 蛋白与特定 RNA 序列的不同程度的关联。
The genomic RNA of negative-strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in Escherichia coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Polyribonucleotides were reencapsidated into the empty NLP, and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.