Generation of novel landomycins M and O through targeted gene disruption
Generation of novel landomycins M and O through targeted gene disruption
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DOI：
10.1002/cbic.200400316
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发表时间：
2005-04-01
期刊：
影响因子：
3.2
通讯作者：
Bechthold, A
中科院分区：
文献类型：
--
作者：
Luzhetskyy, A;Zhu, LL;Bechthold, A
对链霉菌S 136中与兰霉素A生物合成有关的还原糖LonZ 4和羟基糖LonZ 5基因进行了靶向基因失活研究。相应的突变体以及互补实验的分析，使我们能够表明，LanZ 4和LanZ 5是负责独特的C-11-羟基化，发生在土地霉素生物合成。由lanZ 4/Z5突变体积累的化合物是先前描述的兰德霉素F和新的兰德霉素M和O。
Two genes from Streptomyces cyanogenous S 136 that encode the reductose LonZ4 and the hydroxylose LonZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis. Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.