"Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca(2+) fluoroimaging".
"Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca(2+) fluoroimaging".
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DOI：
10.1038/srep21247
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发表时间：
2016-02-16
影响因子：
4.6
通讯作者：
Ohta J
中科院分区：
文献类型：
--
作者：
Kobayashi T;Haruta M;Sasagawa K;Matsumata M;Eizumi K;Kitsumoto C;Motoyama M;Maezawa Y;Ohta Y;Noda T;Tokuda T;Ishikawa Y;Ohta J
为了更好地了解基于神经活动的大脑功能，有必要对自由运动的动物进行微创分析技术。这种技术将为神经科学提供新的知识，并有助于再生医学技术和假肢护理。一种结合光遗传学自动刺激神经、成像可视化神经活动和植入大脑的可穿戴微型仪器的应用可以满足上述需求。为此，本研究新开发了一种可应用于大脑的微创微型装置及其控制系统。由于这种新型植入式装置具有双led和CMOS图像传感器，因此可以同时进行光刺激和荧光成像。这种装置可以通过光与大脑进行双向通信。在本研究中，该装置在体外实验中使用一种新的芯片上3D神经培养细胞外基质凝胶进行了评估，并在体内实验中使用光敏通道和荧光Ca2+指示剂进行了再生医学移植和基因传递到大脑。该装置通过选择性光刺激成功地局部激活细胞，并通过荧光成像同时显示神经细胞的生理Ca2+动力学。
To better understand the brain function based on neural activity, a minimally invasive analysis technology in a freely moving animal is necessary. Such technology would provide new knowledge in neuroscience and contribute to regenerative medical techniques and prosthetics care. An application that combines optogenetics for voluntarily stimulating nerves, imaging to visualize neural activity, and a wearable micro-instrument for implantation into the brain could meet the abovementioned demand. To this end, a micro-device that can be applied to the brain less invasively and a system for controlling the device has been newly developed in this study. Since the novel implantable device has dual LEDs and a CMOS image sensor, photostimulation and fluorescence imaging can be performed simultaneously. The device enables bidirectional communication with the brain by means of light. In the present study, the device was evaluated in an in vitro experiment using a new on-chip 3D neuroculture with an extracellular matrix gel and an in vivo experiment involving regenerative medical transplantation and gene delivery to the brain by using both photosensitive channel and fluorescent Ca2+ indicator. The device succeeded in activating cells locally by selective photostimulation, and the physiological Ca2+ dynamics of neural cells were visualized simultaneously by fluorescence imaging.