Mapping the invisible chromatin transactions of prophase chromosome remodelling
Mapping the invisible chromatin transactions of prophase chromosome remodelling
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绘制前期染色体重塑的不可见染色质变化图
DOI：
10.1101/2021.06.21.449273
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Samejima I
中科院分区：
文献类型：
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作者：
Samejima I
我们已经使用了化学遗传学，染色质蛋白质组学和成像的组合，以映射最早的染色质交易在脊椎动物细胞进入有丝分裂。鸡DT 40 CDK 1as细胞在1 NM-PP 1诱导的G2晚期阻滞释放后15分钟内同步进入有丝分裂。除了染色质与核孔和核膜结合的变化外，最早的前期主要是核糖核蛋白与染色质结合的变化，特别是在核仁中，前rRNA加工因子在RNA聚合酶I之前显著离开染色质。核膜屏障功能在前期早期丧失，细胞质蛋白开始在染色质上积累。结果，外动粒装配似乎通过核膜破裂（NEBD）完成。大多数间期染色质蛋白保持与染色质结合，直到NEBD，之后它们的水平急剧下降。有丝分裂进入期间染色质交易的交互式蛋白质组学图谱可作为https://mitoChEP.bio.ed.ac.uk的资源获得。
We have used a combination of chemical genetics, chromatin proteomics, and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 CDK1ascells undergo synchronous mitotic entry within 15 min following release from a 1NM-PP1-induced arrest in late G2. In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase, and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply. An interactive proteomic map of chromatin transactions during mitotic entry is available as a resource at https://mitoChEP.bio.ed.ac.uk.