ANALYSIS OF THE HUMAN T-CELL RESPONSE TO PICORNAVIRUSES - IDENTIFICATION OF T-CELL EPITOPES CLOSE TO B-CELL EPITOPES IN POLIOVIRUS
ANALYSIS OF THE HUMAN T-CELL RESPONSE TO PICORNAVIRUSES - IDENTIFICATION OF T-CELL EPITOPES CLOSE TO B-CELL EPITOPES IN POLIOVIRUS
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DOI：
10.1128/jvi.67.3.1627-1637.1993
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发表时间：
1993-03-01
影响因子：
5.4
通讯作者：
BORYSIEWICZ, LK
中科院分区：
文献类型：
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作者：
GRAHAM, S;WANG, ECY;BORYSIEWICZ, LK
人类对小核糖核酸病毒的t细胞介导反应的性质和特异性知之甚少。在这项研究中，确定了t细胞对七种小核糖核酸病毒的反应性质，包括脊髓灰质炎病毒、柯萨奇病毒B3和B4、人鼻病毒14和脑心肌炎病毒。29名个体通过T细胞增殖对3型脊髓灰质炎病毒、柯萨奇B3病毒和脑心肌炎病毒有反应，并从这些培养物中建立了130个病毒特异性T细胞系。针对脑心肌炎病毒产生的t细胞系是完全株特异性的。然而，除了脑心肌炎病毒外，大多数针对病毒建立的t细胞系相互之间存在交叉反应。它们的交叉反应性在来自两名受试者的30个小核糖核酸病毒特异性克隆衍生t细胞系中的2个中得到证实，但这些细胞系大多数是血清型特异性的。鉴定了3型脊髓灰质炎病毒VP1中每个b细胞抗原位点附近的t细胞表位。对b细胞抗原位点1附近区域（残基97 ~ 114）的反应在个体间占主导地位。这种主要CD4 t细胞表位的定位可能允许利用天然小核糖核酸病毒t细胞反应构建嵌合病毒，以增加对插入序列特异性抗体的产生。
Little is known about the nature and specificity of T-cell-mediated responses to picornaviruses in humans. In this study, the nature of the T-cell response to seven picornaviruses, including polioviruses, coxsackieviruses B3 and B4, human rhinovirus 14, and encephalomyocarditis virus was determined. Twenty-nine individuals responded to poliovirus type 3, coxsackievirus B3, and encephalomyocarditis virus by proliferation of T cells, and from such cultures, 130 virus-specific T-cell lines were established. T-cell lines generated in response to encephalomyocarditis virus were exclusively strain specific. However, the majority of T-cell lines established in response to viruses, other than encephalomyocarditis virus, were cross-reactive to each other. Their cross-reactivity was confirmed in 2 of the 30 picornavirus-specific clonally derived T-cell lines from two subjects, but the majority of these lines were serotype specific. T-cell epitopes adjacent to each of the B-cell antigenic sites in VP1 of poliovirus type 3 were identified. The response to the region adjacent to B-cell antigenic site 1 (residues 97 to 114) was dominant between individuals. The localization of this major CD4 T-cell epitope may permit the construction of chimeric viruses utilizing the natural picornavirus T-cell response to augment production of antibody specific for inserted sequences.