Potential for interdependent development of tRNA determinants for aminoacylation and ribosome decoding.
Potential for interdependent development of tRNA determinants for aminoacylation and ribosome decoding.
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DOI：
10.1038/ncomms1331
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发表时间：
2011
影响因子：
16.6
通讯作者：
Hou, Ya-Ming
中科院分区：
文献类型：
--
作者：
Liu, Cuiping;Gamper, Howard;Liu, Hanqing;Cooperman, Barry S.;Hou, Ya-Ming
虽然tRNA中氨基酰化所需的核苷酸在进化过程中是保守的，但细菌的氨基酰-tRNA合成酶（aaRSs）不能对真核tRNA进行酰基化。跨物种屏障可能是由于细菌aars缺乏真核特异性结构域。在这里，我们发现虽然大肠杆菌CysRS不能酰基化人类tRNACys，但人类CysRS的真核特异性结构域的融合克服了人类tRNACys的跨物种屏障。除了能够识别tRNACys三级核心的序列差异外，融合的真核结构域还将大肠杆菌CysRS的特异性从细菌tRNACys中的A37重定向到哺乳动物中的G37。进一步的实验表明，核糖体上密码子识别的准确性对tRNACys中a37到g37的转换也高度敏感。这些结果提高了tRNA氨基酰化核苷酸决定因素与核糖体解码决定因素相互依赖的可能性。
Although the nucleotides in tRNA required for aminoacylation are conserved in evolution, bacterial aminoacyl-tRNA synthetases (aaRSs) are unable to acylate eukaryotic tRNA. The cross-species barrier may be due to the absence of eukaryote-specific domains from bacterial aaRSs. Here we show that while E. coli CysRS cannot acylate human tRNACys, the fusion of a eukaryote-specific domain of human CysRS overcomes the cross-species barrier in human tRNACys. In addition to enabling recognition of the sequence differences in the tertiary core of tRNACys, the fused eukaryotic domain redirects the specificity of E. coli CysRS from the A37 present in bacterial tRNACys to the G37 in mammals. Further experiments show that the accuracy of codon recognition on the ribosome was also highly sensitive to the A37-to-G37 transition in tRNACys. These results raise the possibility of the development of tRNA nucleotide determinants for aminoacylation being interdependent with those for ribosome decoding.
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影响因子：
56.9
作者：
Ogle, JM;Brodersen, DE;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
影响因子：
4.8
作者：
Hauenstein, Scott I.;Hou, Ya-Ming;Perona, John J.
通讯作者：
Perona, John J.
DOI：
10.1073/pnas.90.14.6776
发表时间：
1993-07-15
影响因子：
11.1
作者：
HOU, YM;WESTHOF, E;GIEGE, R
通讯作者：
GIEGE, R
影响因子：
2.9
作者：
HOU, YM
通讯作者：
HOU, YM
影响因子：
64.8
作者：
LEVITT, M
通讯作者：
LEVITT, M