Methanogenesis from Sucrose by Defined Immobilized Consortia
Methanogenesis from Sucrose by Defined Immobilized Consortia
复制标题
通过固定化联合体从蔗糖中产甲烷
DOI：
10.1128/aem.47.1.1-6.1984
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发表时间：
1984
影响因子：
4.4
通讯作者：
R. Wolfe
中科院分区：
文献类型：
--
作者：
W. J. Jones;J. Guyot;R. Wolfe
构建了一个能够将蔗糖主要降解为 CH4 和 CO2 的细菌群落，其中乙酸盐作为关键的产甲烷前体。此外，还确定了琼脂固定对聚体活性的影响。主要发酵生物体大肠杆菌产生乙酸盐、甲酸盐、H2 和 CO2（已知的产甲烷菌底物），以及乙醇和乳酸，这些化合物不是产甲烷菌的底物。非产甲烷底物乳酸和乙醇氧化成乙酸盐是通过添加伍氏醋杆菌和脱硫弧菌介导的。产甲烷阶段是通过添加嗜乙酸产甲烷菌巴氏甲烷八叠球菌和嗜氢产甲烷菌甲酸甲烷杆菌来完成的。使用低底物浓度（0.05 至 0.2% [wt/vol]）、生长限制培养基和五组分联合体的研究结果表明，蔗糖碳可有效转化（40%）为 CH4。如果省略该联合体的任何组成部分，就会观察到 CH4 产量和 CH4 生产速率显着下降。大约 70% 的 CH4 是通过乙酸盐产生的。该联合体的琼脂固定化细胞表现出与非固定化细胞相似的 CH4 产量和从蔗糖产生 CH4 的速率。当反应条件中省略半胱氨酸时，CH4 生成率下降 25%；当固定化聚体在 4°C 下保存 1 周时，CH4 生成率下降 40%。
A bacterial consortium capable of sucrose degradation primarily to CH4 and CO2 was constructed, with acetate as the key methanogenic precursor. In addition, the effect of agar immobilization on the activity of the consortium was determined. The primary fermentative organism, Escherichia coli, produced acetate, formate, H2, and CO2 (known substrates for methanogens), as well as ethanol and lactate, compounds that are not substrates for methanogens. Oxidation of the nonmethanogenic substrates, lactate and ethanol, to acetate was mediated by the addition of Acetobacterium woodii and Desulfovibrio vulgaris. The methanogenic stage was accomplished by the addition of the acetophilic methanogen Methanosarcina barkeri and the hydrogenophilic methanogen Methanobacterium formicicum. Results of studies with low substrate concentrations (0.05 to 0.2% [wt/vol]), a growth-limiting medium, and the five-component consortium indicated efficient conversion (40%) of sucrose carbon to CH4. Significant decreases in yields of CH4 and rates of CH4 production were observed if any component of the consortium was omitted. Approximately 70% of the CH4 generated occurred via acetate. Agar-immobilized cells of the consortium exhibited yields of CH4 and rates of CH4 production from sucrose similar to those of nonimmobilized cells. The rate of CH4 production decreased by 25% when cysteine was omitted from reaction conditions and by 40% when the immobilized consortium was stored for 1 week at 4°C.