Antimicrobial peptide induction in the haemolymph of the Mexican scorpion Centruroides limpidus limpidus in response to septic injury
Antimicrobial peptide induction in the haemolymph of the Mexican scorpion Centruroides limpidus limpidus in response to septic injury
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DOI：
10.1007/s00018-004-4096-x
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发表时间：
2004-06-01
影响因子：
8
通讯作者：
Possani, LD
中科院分区：
文献类型：
--
作者：
de la Vega, RCR;García, BI;Possani, LD
抗菌肽(AMPs)是宿主抵御感染微生物的重要成分。在螯合生物中，它们参与了三种不同的防御系统：一种是编码AMPs的基因立即上调，另一种是AMPs从细胞储藏中诱导的系统性释放，第三种替代是AMPs的系统性结构性生产。在这项工作中，我们使用了差示高效液相色谱和质谱仪的方法来表明败血症损伤在墨西哥蝎血淋巴中引起了免疫反应。我们分离了几种血淋巴成分，其中一种被广泛鉴定(氨基酸序列、二硫键配对、cDNA和基因组克隆)，并被证明是无脊椎动物防御素家族的一个新成员，因此被命名为林氏隐翅虫防御素样肽(CLL-DLP)。这种多肽在血淋巴中积聚，以响应败血症的损伤，独立于转录调节。
Antimicrobial peptides (AMPs) are essential components of host defences against infectious microrganisms. In chelicerate organisms they have been implicated in three alternative defensive systems: one is defined by the immediate up-regulation of genes encoding AMPs, another is characterized by the inducible systemic release of AMPs from cellular reservoirs and the third alternative is the systemic constitutive production of AMPs. In this work we used a differential high-performance liquid chromatography and mass spectrometry approach to show that septic injury elicits an immune response in the haemolymph of the Mexican scorpion Centruroides limpidus limpidus. We isolated several haemolymph components, one of which was characterized extensively (amino acid sequence, disulphide pairing, cDNA and genomic clones) and demonstrated to be a novel member of the invertebrate defensin family and consequently named C. limpidus limpidus defensin-like peptide (Cll-dlp). This peptide accumulates in the haemolymph in response to septic injury, independently of transcriptional regulation.