Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene.
Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene.
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可育和雄性不育向日葵线粒体 DNA 的物理和基因组织。
DOI：
10.1093/nar/16.9.3787
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发表时间：
1988
影响因子：
14.9
通讯作者：
Palmer,JD
中科院分区：
文献类型：
--
作者：
Siculella,L;Palmer,JD
为了研究向日葵（Helianthus annuus）细胞质雄性不育（CMS）的分子基础，我们比较了同核可育系和 CMS 系线粒体 DNA（mtDNA）的物理组织和转录特性。绘图研究显示，与之前对其他植物物种的可育系和 CMS 系的比较相比，这两种 mtDNA 之间的相似性要大得多。两个向日葵 mtDNA 1) 大小几乎相同（300 kb 和 305 kb）； 2) 含有相同的12 kb重组重复序列和相关的三联结构； 3) 具有相同的分散分布的线粒体基因和叶绿体DNA同源序列； 4) 一级序列同一性大于99.9%； 5) 在包含 94% 基因组的连续区域上共线。可检测到的改变仅限于基因组的 17 kb 区域，并且仅反映两个突变 - 12 kb 倒置和 5 kb 插入/删除。两种重排的一个终点位于 atpA 内或附近，这也是唯一一个其转录本在可育系和 CMS 系之间存在差异的线粒体基因。此外，恢复 CMS 植物育性的核基因特异性影响 atpA 转录本的模式。因此，theatpA 基因座的重排可能是向日葵中 CMS 的原因。
To study the molecular basis of cytoplasmic male sterility (CMS) in sunflower (Helianthus annuus), we compared the physical organization and transcriptional properties of mitochondrial DNAs (mtDNAs) from isonuclear fertile and CMS lines. Mapping studies revealed much greater similarity between the two mtDNAs than in previous comparisons of fertile and CMS lines from other plant species. The two sunflower mtDNAs 1) are nearly identical in size (300 kb and 305 kb); 2) contain the same 12 kb recombination repeat and associated tripartite structure; 3) have the same dispersed distribution of mitochondrial genes and chloroplast DNA-homologous sequences; 4) are greater than 99.9% identical in primary sequence; and 5) are colinear over a contiguous region encompassing 94% of the genome. Detectable alterations are limited to a 17 kb region of the gename and reflect as few as two mutations - a 12 kb inversion and a 5 kb insertion/deletion. One endpoint of both rearrangements is located within or nearatpA, which is also the only mitochondrial gene whose transcripts differ between the fertile and CMS lines. Furthermore, a nuclear gene that restores fertility to CMS plants specifically influences the pattern ofatpA transcripts. Rearrangements at theatpA locus may, therefore, be responsible for CMS in sunflower.