Evidence for a multifocal origin of papillary serous carcinoma of the peritoneum.
Evidence for a multifocal origin of papillary serous carcinoma of the peritoneum.
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发表时间：
1995-02
期刊：
影响因子：
11.2
通讯作者：
M. Muto;William R. Welch;Samuel C. Mok;C. Bandera;Paula M. Fishbaugh;S. Tsao;Ching C. Lau;Howard M. Goodman;Robert C. Knapp;Ross S. Berkowitz
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作者：
M. Muto;William R. Welch;Samuel C. Mok;C. Bandera;Paula M. Fishbaugh;S. Tsao;Ching C. Lau;Howard M. Goodman;Robert C. Knapp;Ross S. Berkowitz
组织学证据表明，乳头状浆液性腹膜癌（PSCP）可能是多灶性起源。应用PCR方法检测福尔马林固定组织中的串联重复序列多态性，研究了6例PSCP患者第1、3、4和17号染色体上8个位点的杂合性丢失。杂合性缺失在5至11个肿瘤部位/患者进行评估。在1 q32-qter、3p14.3-21.1、17 q12、17q21.3-23等4个位点上观察到等位基因丢失。3例患者在不同的解剖部位表现出不同的等位基因丢失模式。在另外一个病例中，通过定量PCR和单链构象多态性分析检测到p53基因突变，仅在5个肿瘤部位中的2个中检测到。6例PSCP中有4例在同一患者的肿瘤部位等位基因丢失和p53基因突变模式不同。这些发现与组织病理学证据一致，即PSCP是多灶性起源的。
Histopathological evidence suggests that papillary serous carcinoma of the peritoneum (PSCP) may be multifocal in origin. Utilizing a PCR based method to detect tandem repeat polymorphisms in formalin fixed tissue, loss of heterozygosity at eight loci on chromosomes 1, 3, 4, and 17 was studied in six cases of PSCP. Loss of heterozygosity was assessed at between 5 and 11 tumor sites/patient. Allelic losses at 4 loci (1q32-qter, 3p14.3-21.1, 17q12, 17q21.3-23) were noted. Three cases demonstrated a different pattern of allelic loss at various anatomic sites within the same patient. In an additional case, a mutation of the p53 gene, detected by quantitative PCR followed by single-strand conformation polymorphism analysis, was detected in only 2 of 5 tumor sites. The pattern of allelic loss and the mutational pattern of the p53 gene varied at tumor sites within the same patient in 4 of 6 cases of PSCP. These findings are consistent with histopathological evidence that PSCP is multifocal in origin.