TACHYKININS INDUCE SECRETION OF PROLACTIN FROM PERIFUSED RAT ANTERIOR-PITUITARY-CELLS BY INTERACTIONS WITH 2 DIFFERENT BINDING-SITES
TACHYKININS INDUCE SECRETION OF PROLACTIN FROM PERIFUSED RAT ANTERIOR-PITUITARY-CELLS BY INTERACTIONS WITH 2 DIFFERENT BINDING-SITES
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DOI：
10.3109/10799899509045238
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
MAU, SE
中科院分区：
文献类型：
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作者：
HENRIKSEN, JS;SAERMARK, T;MAU, SE
P物质和其他两种哺乳动物速激肽，神经激肽A和B，被认为在垂体前叶水平具有直接调节作用。我们已经研究了P物质（SP）和神经激肽B（NKB），单独和组合，从培养的垂体前叶细胞上生长的胶原蛋白包被的微珠，并放置在灌注系统中的催乳素释放的影响。催乳素（Prl）分泌后25秒内观察到暴露于任一促分泌素，并达到最大值在60-80秒。此外，SP和NKB诱导的催乳素反应具有剂量依赖性。当SP或NKB灌流时，PRL分泌保持恒定长达4小时，然后逐渐下降至基础水平。同时添加次最大浓度的SP和NKB导致相加反应相比，无论是单独的促分泌素的反应。连续（8小时）灌流与SP没有阻止正常的催乳素反应NKB或TRH。这些结果表明，速激肽，P物质和神经激肽B，释放Pr 1从灌流的雌性大鼠垂体前叶细胞与两种不同的受体，可能是NK 1和NK 3速激肽受体亚型的相互作用。
Substance P and the two other mammalian tachykinins, neurokinin A and B, are accepted to have direct regulating effects at the anterior pituitary level. We have examined the effects of substance P (SP) and neurokinin B (NKB), alone and in combination, on prolactin release from cultured anterior pituitary cells grown on collagen-coated micro beads and placed in a perfusion system. Prolactin (Prl) secretion was observed within 25 s after exposure to either secretagogue and reached a maximum within 60-80 s. Furthermore, the prolactin response induced by SP and NKB was dose-dependent. Prl secretion remained constant for up to 4 h when SP or NKB were perifused and then fell gradually towards basal levels. Simultaneous addition of submaximal concentrations of SP and NKB resulted in an additive response compared with the responses of either secretagogue alone. Continuous (8 h) perifusion with SP did not prevent a normal prolactin response by NKB or TRH. These results indicate that the tachykinins, substance P and neurokinin B, release Prl from perifused female rat anterior pituitary cells by interaction with two different receptors, possibly the NK1 and NK3 tachykinin receptor subtypes.