Tracing Axons in the Peripheral Nerve Using LacZ Gene Recombinant Adenovirus and Its Application to Regeneration of the Peripheral Nerve
Tracing Axons in the Peripheral Nerve Using LacZ Gene Recombinant Adenovirus and Its Application to Regeneration of the Peripheral Nerve
复制标题
LacZ基因重组腺病毒示踪周围神经轴突及其在周围神经再生中的应用
DOI：
10.1093/jnen/60.7.671
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发表时间：
2001
影响因子：
3.2
通讯作者：
S. Hirano
中科院分区：
文献类型：
--
作者：
Hitoshi Miwa;M. Shibata;H. Okado;S. Hirano
研究了带LacZ的重组腺病毒对外周神经系统轴突的追踪作用。将带LacZ的重组腺病毒应用于大鼠胫骨神经切端。LacZ基因产物（ß-半乳糖苷酶）填充胫骨神经轴突，使轴突的连续远程追踪成为可能。此外，分支和分支的方向也可以被检查。胫骨神经中的标记轴突彼此平行，没有分支，并在胫骨和坐骨神经中保持这种相对位置。当用硅管将病毒注入再生神经时，再生纤维扭曲地生长，在硅管近端凸起处有短分支。纤维在管中直直地生长，并穿过管的远端凸起而没有分支。这些观察结果表明，LacZ基因重组腺病毒是研究周围神经系统和再生过程的有用示踪剂。
The usefulness of recombinant adenovirus with LacZ to trace axons in the peripheral nervous system was investigated. Recombinant adenovirus with LacZ was applied to the cut end of the tibial nerve in rats. The LacZ gene product (ß-galactosidase) filled axons of the tibial nerve, which permitted the continuous long-range tracing of axons. Further, the branching and the direction of the branches could also be examined. Labeled axons in the tibial nerves ran parallel to each other without branching and kept this relative position in the tibial and the sciatic nerve. When the virus was introduced to the regenerating nerve using a silicon tube, the regenerating fibers grew tortuously with short branches in the bulge at the proximal end of the silicon tube. The fibers grew straight in the tube and passed through the bulge at the distal end of the tube without branching. These observations indicate that the LacZ gene recombinant adenovirus is a useful tracer for the study of the peripheral nervous system and of the regeneration processes.