CYP94A1, a plant cytochrome P450-catalyzing fatty acid ω-hydroxylase, is selectively induced by chemical stress in Vicia sativa seedlings
CYP94A1, a plant cytochrome P450-catalyzing fatty acid ω-hydroxylase, is selectively induced by chemical stress in Vicia sativa seedlings
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DOI：
10.1007/s00425-005-1503-y
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发表时间：
2005-08-01
期刊：
影响因子：
4.3
通讯作者：
Pinot, F
中科院分区：
文献类型：
--
作者：
Benveniste, I;Bronner, R;Pinot, F
CYP 94 A1是一种细胞色素P450（P450），催化蚕豆幼苗脂肪酸（FA）ω-羟基化。为了研究这FA单加氧酶的生理作用，我们在这里报告其在转录水平上的调控（北方印迹）。CYP 94 A1和另外两种依赖于P450的FA羟化酶（CYP 94 A2和CYP 94 A3）的转录本在幼苗的早期发育过程中几乎检测不到。CYP 94 A1转录本与其他两种亚型的转录本相比，（少于20分钟）和强烈（超过100倍）增强治疗后，氯贝特，一种降血脂药物在动物和抗生长素（p-氯苯氧基异丁酸）在植物中，由生长素（2，4-二氯苯氧基乙酸和吲哚-3-乙酸）、无活性的生长素类似物（2，3-二氯苯氧基乙酸）以及水杨酸。所有这些化合物仅在高浓度（50-500 μ M范围）下激活CYP 94 A1转录。平行地，这些高水平的氯贝特和生长素改变幼苗生长和发育。因此，CYP 94 A1在这些条件下的表达和伴随的形态学和细胞学修饰将表明该P450在植物防御化学损伤的过程中的含义。
CYP94A1 is a cytochrome P450 (P450) catalyzing fatty acid (FA) omega-hydroxylation in Vicia sativa seedlings. To study the physiological role of this FA monooxygenase, we report here on its regulation at the transcriptional level (Northern blot). Transcripts of CYP94A1, as those of two other P450-dependent FA hydroxylases (CYP94A2 and CYP94A3) from V. sativa, are barely detectable during the early development of the seedlings. CYP94A1 transcripts, in contrast to those of the two other isoforms, are rapidly (less than 20 min) and strongly (more than 100 times) enhanced after treatment by clofibrate, an hypolipidemic drug in animals and an antiauxin (p-chlorophenoxyisobutyric acid) in plants, by auxins (2,4-dichlorophenoxyacetic acid and indole-3-acetic acid), by an inactive auxin analog (2,3-dichlorophenoxyacetic acid), and also by salicylic acid. All these compounds activate CYP94A1 transcription only at high concentrations (50-500 mu M range). In parallel, these high levels of clofibrate and auxins modify seedling growth and development. Therefore, the expression of CYP94A1 under these conditions and the concomitant morphological and cytological modifications would suggest the implication of this P450 in a process of plant defense against chemical injury.