QTL mapping for flowering time in different latitude in soybean
QTL mapping for flowering time in different latitude in soybean
复制标题
大豆不同纬度开花时间的QTL定位
DOI：
10.1007/s10681-015-1501-5
复制
发表时间：
2015-12-01
期刊：
影响因子：
1.9
通讯作者：
Kong, Fanjiang
中科院分区：
文献类型：
--
作者：
Lu, Sijia;Li, Ying;Kong, Fanjiang
开花标志着植物从营养阶段向生殖阶段的过渡，在许多农艺性状中起着重要作用。对于大豆这种短日照诱导的光周期敏感植物来说，延迟短日照环境下的开花时间是非常重要的，并已被育种者用来提高产量和增强植物在低纬度地区的适应性。本研究的目的是鉴定与开花时间相关的数量性状位点（qtl），特别是与SD环境下延迟开花时间的长幼子性状（LJ）相关的qtl。利用AGS292与K3杂交获得的91个重组自交系群体进行图谱构建和QTL分析。该图谱覆盖2546.7 cM，包含52个启动子特异性indel和9个外显子特异性indel标记。在9种环境中，包括4种短日照（低纬度）环境和5种长日照（高纬度）环境，检测了表型日数到开花的数据。在SD环境中，检测到6个qtl。其中5个与LJ特质有关。在5个LJ qtl中，有4个qtl可能归属于已知的开花时间基因，分别为FT5a位点的qFT-J-1、FT2a位点的qFT-J-2、E2位点的qFT-O和E3位点的qFT-L。这是首次报道E2、E3、FT2a和FT5a位点可能与LJ性状相关。在5个LD环境下，E2位点的qFT-O和E3位点的qFT-L与预期一致，表明E2和E3位点对大豆在LD光期的适应非常重要。多环境联合分析鉴定出9对加性qtl和9对上位性qtl，其中大部分与环境相互作用有关。共鉴定出5个可能代表开花时间基因的qtl （qFT-B2-1、qFT-C1-1、qFT-K、qFT-D2和qFT-F）。这为今后大豆花期的精细定位、图谱克隆和分子辅助育种研究奠定了基础。
Flowering represents the transition from the vegetative to reproductive phase and plays an important role in many agronomic traits. For soybean, a short day (SD) induced and photoperiod-sensitive plant, delaying flowering time under SD environments is very important and has been used by breeders to increase yields and enhance plant adaptabilities at lower latitudes. The purpose of this study was to identify quantitative trait loci (QTLs) associated with flowering time, especially QTLs underlying the long juvenile (LJ) trait which delays flowering time under SD environments. A population of 91 recombinant inbred lines derived from a cross between AGS292 and K3 was used for map construction and QTL analysis. The map covered 2546.7 cM and included 52 new promoter-specific indel and 9 new exon-specific indel markers. The phenotypic days-to-flowering data were examined in nine environments, including four short-day (SD, low latitude) and five long-day photoperiod (LD, high latitude) environments. For the SD environments, six QTLs were detected. Five of them were associated with the LJ trait. Among the five LJ QTLs, four QTLs may be attributed to the known flowering time genes, including qFT-J-1 for FT5a locus, qFT-J-2 for the FT2a locus, qFT-O for the E2 locus and qFT-L for the E3 locus. This is the first report that the E2, E3, FT2a and FT5a loci may be associated with the LJ trait. Under the five LD environments, as expected, qFT-O for the E2 locus and qFT-L for the E3 locus were identified, suggesting that E2 and E3 loci are very important for soybean adaptation in LD photoperiod. Conjoint analysis of multiple environments identified nine additive QTLs and nine pairs of epistatic QTLs, among which most were involved in interactions with the environments. In total, five QTLs (qFT-B2-1, qFT-C1-1, qFT-K, qFT-D2 and qFT-F) were identified that may represent novel flowering time genes. This provides a fundamental foundation for future studies of flowering time in soybean using fine mapping, map-based cloning, and molecular-assisted breeding.