Maintenance of separated rat pituitary mammotrophs in cell culture.
Maintenance of separated rat pituitary mammotrophs in cell culture.
复制标题
在细胞培养物中维持分离的大鼠垂体乳房营养细胞。
DOI：
10.1210/endo-98-1-25
复制
发表时间：
1976
期刊：
影响因子：
4.8
通讯作者：
W. Hymer
中科院分区：
文献类型：
--
作者：
J. Snyder;W. Wilfinger;W. Hymer
将分散的雌性大鼠垂体前叶细胞在含有20%胎牛血清的培养基199中培养30天。在此期间，培养基中的催乳素水平保持相对恒定，范围为30-40 ng/10,000个细胞/天。释放到培养基中的催乳素总量是细胞原含量的10-15倍。乳腺营养细胞的形态完整性得以保持。使用单位重力流速沉淀法，将未经处理、过度切除或雌激素激发的动物细胞分离成几个部分，随后培养14天。在此期间，并非所有乳腺肥大细胞分泌的激素数量相同。这些数据表明，雌性大鼠的垂体是由异质的乳腺营养细胞组成的，它们在体外分泌催乳素的能力可能在一定程度上反映了动物先前的生理状态。
Dispersed female rat anterior pituitary cells were cultured in Medium 199 containing 20% fetal calf serum for 30 days. Prolactin levels in the culture medium remained relatively constant during this time, ranging from 30-40 ng/10,000 cells seeded/day. The total quantity of prolactin released into the medium was 10-15 times that originally contained in the cells. Morphological integrity of the mammotrophs was maintained. Using velocity sedimentation at unit gravity, cells from untreated, overiectomized or estrogen-primed animals were separted into several fractions, and subsequently cultured for 14 days. Not all mammotrophs secreted the same quantity of hormone during this time. The data suggest that the pituitary of the female rat is composed of a heterogeneous population of mammotrophs, and that their capacity to secrete prolactin in vitro may, in part, be reflected by the previous physiological status of the animal.