Neonatal tactile stimulation decreases depression-like and anxiety-like behaviors and potentiates sertraline action in young rats
Neonatal tactile stimulation decreases depression-like and anxiety-like behaviors and potentiates sertraline action in young rats
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DOI：
10.1016/j.ijdevneu.2015.09.010
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发表时间：
2015-12-01
影响因子：
1.8
通讯作者：
Burger, Marilise Escobar
中科院分区：
文献类型：
--
作者：
Freitas, Daniele;Antoniazzi, Caren T. D.;Burger, Marilise Escobar
众所周知，生命早期发生的事件对大脑发育产生重大影响，这些影响可以反映在整个成年期。本研究旨在评估新生儿触觉刺激（TS）对与抑郁样和焦虑样行为相关的行为和形态学反应的影响，并在给予选择性5-羟色胺再摄取抑制剂（SSRI）舍曲林（SERT）后进行评估。雄性幼仔从出生后第8天（PND 8）至出生后第14天（PND 14）接受每日TS，每天持续10分钟。在PND 50，成年动物接受强迫游泳训练（15分钟）。在PND 51，每个实验组（UH和TS）的一半接受单次亚治疗剂量的舍曲林（SER，0.3mg/kg体重，i. p.）或其媒介物（C，对照组）。注射后30分钟，在强迫游泳试验（FST，5分钟）中量化抑郁样行为。第二天，在高架十字迷宫（高架十字迷宫）中评估焦虑样行为，然后进行生化评估。TS本身增加游泳时间，减少不动时间在FST。此外，TS本身能够增加头部下降的频率和在张开的手臂中花费的时间，从而降低焦虑指数。此外，暴露于TS的组显示皮质酮本身的血浆水平降低。有趣的是，虽然TS暴露显著增强了亚治疗剂量的SEAT的抗抑郁活性，但这种药物能够加剧TS诱导的抗焦虑活性，分别在FST和ESTA中观察到。暴露于TS并用SERT治疗的动物中皮质酮和皮质醇的血浆水平降低能够证实这种新生儿处理与抗抑郁药物之间的有趣相互作用。从我们的研究结果，我们得出结论，新生儿TS是能够发挥有益的影响能力，以科普压力的情况下，在成年期，预防抑郁症和有利地调节抗抑郁药物的作用。(C)2015爱思唯尔有限公司版权所有。
It is well known that events which occur in early life exert a significant influence on brain development, what can be reflected throughout adulthood. This study was carried out in order to assess the influence of neonatal tactile stimulation (TS) on behavioral and morphological responses related to depression-like and anxiety-like behaviors, assessed following the administration of sertraline (SERT), a selective serotonin re-uptake inhibitor (SSRI). Male pups were submitted to daily TS, from postnatal day 8 (PND8) to postnatal day 14 (PND14), for 10 min every day. On PND50, adult animals were submitted to forced swimming training (15 min). On PND51, half of each experimental group (UH and TS) received a single sub-therapeutic dose of sertraline (SER, 0.3 mg/kg body weight, i.p.) or its vehicle (C, control group). Thirty minutes after injection, depression-like behaviors were quantified in forced swimming test (FST, for 5 min). On the following day, anxiety-like behaviors were assessed in elevated plus maze (EPM), followed by biochemical assessments. TS per se increased swimming time, decreasing immobility time in FST. Besides, TS per se was able to increase frequency of head dipping and time spent in the open arms of EPM, resulting in decreased anxiety index. In addition, groups exposed to TS showed decreased plasma levels of corticosterone per se. Interestingly, while TS exposure significantly potentiated the antidepressant activity of a subtherapeutic dose of SEAT, this drug was able to exacerbate TS-induced anxiolytic activity, as observed in FST and EPM, respectively. Decreased plasma levels of both corticosterone and cortisol in animals exposed to TS and treated with SERT are able to confirm the interesting interaction between this neonatal handling and the antidepressant drug. From our results, we conclude that neonatal TS is able to exert beneficial influence on the ability to cope with stressful situations in adulthood, preventing depression and favorably modulating the action of antidepressant drugs. (C) 2015 Elsevier Ltd. All rights reserved.