Identical epidermal growth factor receptor mutations in adenocarcinomatous and squamous cell carcinomatous components of adenosquamous carcinoma of the lung
Identical epidermal growth factor receptor mutations in adenocarcinomatous and squamous cell carcinomatous components of adenosquamous carcinoma of the lung
复制标题
DOI：
10.1002/cncr.22413
复制
发表时间：
2007-02-01
期刊：
影响因子：
6.2
通讯作者：
Chang, Joon
中科院分区：
文献类型：
--
作者：
Kang, Shin Myung;Kang, Hyun Ju;Chang, Joon
背景肺腺鳞癌由腺癌和鳞状细胞癌组成。表皮生长因子受体（EGFR）基因突变多见于肺腺癌，在肺鳞癌中少见。探讨EGFR在腺鳞癌各组成部分中的突变状态，并根据突变状态对患者进行定性，有助于了解腺鳞癌的组织发生。采用聚合酶链反应-单链构象多态性技术，对25例韩国腺鳞癌患者的EGFR激酶结构域第18 ~ 21外显子的突变状态进行了研究。根据EGFR突变状态比较患者的临床病理特征。在11例（44%）患者中发现EGFR突变：9例突变位于外显子19，1例位于外显子20，1例位于外显子21。EGFR突变在女性（n = 8，80%）中的发生率显著高于男性（n = 3，20%）（P = 0.005）。不吸烟者（n = 8，62%）的EGFR突变比吸烟者（n = 3，25%; P = 0.111）更常见。核苷酸测序证实腺鳞癌的两个组分中EGFR突变相同。EGFR突变的频率和EGFR突变体在腺鳞癌中的临床病理特征与亚洲腺癌患者相似。相同的EGFR突变在这两个组成部分表明单克隆性的可能性在腺鳞癌的组织发生。
BACKGROUND. Adenosquamous carcinoma of the lung is composed of adenocarcinomatous and squamous cell carcinomatous components. The epidermal growth factor receptor (EGFR) mutations occur mostly in adenocarcinomas and rarely in squamous cell carcinoma of lung. Attempts to investigate the EGFR mutation status in each component of adenosquamous carcinoma and to characterize the patients according to mutation status may help to understand the histogenesis of adenosquamous carcinoma.METHODS. The mutation status of EGFR kinase domain from exon 18 to 21 was investigated in 25 Korean patients with adenosquamous carcinoma by polymerase chain reaction-single strand conformation polymorphism using the tissues of each component from the adenosquamous carcinoma tumor. Clinicopathologic characteristics of the patients according to the status of EGFR mutations were compared.RESULTS. EGFR mutations were identified in 11 (44%) patients: 9 mutations were in exon 19, 1 in exon 20, and I in exon 21. EGFR mutations were significantly more frequent (P = .005) in women (n = 8, 80%) than men (n = 3, 20%). Never-smokers (n = 8, 62%) had EGFR mutations more commonly than smokers (n = 3, 25%; P = .111). Identical EGFR mutations in both components of adenosquamous carcinoma were confirmed by nucleotide sequencing.CONCLUSIONS. The frequency of EGFR mutation and clinicopathologic characteristics of the EGFR mutants in adenosquamous carcinoma are similar to those of Asian patients with adenocarcinomas. Identical EGFR mutations in both components suggest the possibility of monoclonality in the histogenesis of adenosquamous carcinoma.