RNA sequencing analysis of the developing chicken retina
RNA sequencing analysis of the developing chicken retina
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DOI：
10.1038/sdata.2016.117
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发表时间：
2016-12-20
期刊：
影响因子：
9.8
通讯作者：
Enke, Raymond A.
中科院分区：
文献类型：
--
作者：
Langouet-Astrie, Christophe J.;Meinsen, Annamarie L.;Enke, Raymond A.
使用大规模并行的下一代测序技术的RNA测序转录组分析提供了了解整个组织样品范围内基因表达的全局变化的能力。脊椎动物视网膜的发育需要复杂的转录激活和抑制的时间编排。鸡胚胎是研究发育生物学和视网膜发生的经典模型系统。现有的视网膜转录组项目对于视觉研究界研究小鼠和人类视网膜发生的各个方面至关重要，然而，目前还没有公开的数据集描述发育中的鸡视网膜转录组。在这里，我们使用Illumina RNA测序（RNA-seq）分析来表征发育中的鸡视网膜的mRNA转录组，以鉴定这种重要模式生物中对视网膜发育至关重要的基因。这些数据将是有价值的视觉研究界表征全球变化的基因表达之间的眼组织和关键的发育时间点在鸡视网膜的视网膜发生。
RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of the developing chicken retina in an effort to identify genes critical for retinal development in this important model organism. These data will be valuable to the vision research community for characterizing global changes in gene expression between ocular tissues and critical developmental time points during retinogenesis in the chicken retina.